Sen Gupta A K
Z Lebensm Unters Forsch. 1975 Jun 30;158(2):71-2. doi: 10.1007/BF01460022.
A rapid method for routine determination of oil in commercial lecithins and similar phospholipid samples has been worked out. This based on the observation that under the described experimental conditions 1 g of phospholipids is completely adsorbed on 45 g silica gel which has previously been disactivated by the addition of 15% water. The lecithin sample is filtered in ethereal solution through a column filled with disactivated silica gel which is subsequently washed with a little ether. The combined ether eluates are evaporated to dryness invacuo and the residue weighed. This residue is the oil part of the sample and is constituted of mono-, di-, and triglycerides, free fatty acids, sterols and their derivatives. The duration for one such determination is about 20 min.
已研发出一种快速测定商业卵磷脂及类似磷脂样品中油含量的常规方法。该方法基于以下观察结果：在所描述的实验条件下，1克磷脂完全吸附在45克硅胶上，该硅胶先前已通过添加15%的水进行了去活化处理。卵磷脂样品在乙醚溶液中通过填充有去活化硅胶的柱子进行过滤，随后用少量乙醚洗涤。合并的乙醚洗脱液在真空中蒸发至干，然后称量残留物。该残留物即为样品的油部分，由单甘油酯、二甘油酯、三甘油酯、游离脂肪酸、甾醇及其衍生物组成。一次这样的测定所需时间约为20分钟。
