[通过硅胶薄层层析法测定高密度脂蛋白的脂质组成]

[Determination of lipid composition of high density lipoproteins by thin-layer chromatography on silica gel].

作者信息

Tvorogova M G, Isaeva E L, Prokazova N V, Rozhkova T A, Kukharchuk V V, Titov V N

出版信息

Klin Lab Diagn. 1998 Apr(4):13-6.

Abstract

Unidimensional chromatography in a thin layer of silica gel with two solvent systems for separation of the lipid extract of high density lipoproteins (HDLP) detected 10 lipid fractions in subjects with normal lipidemia. Four phospholipid fractions were identified: three main (phosphatidylcholine, lysophosphatidylcholine, and sphingomyelin) and one minor, phosphatidylethanolamine. HDLP lipids include free cholesterol and its esters; three glyceride fractions are detected: mono- di, and triglycerides, and free fatty acids. The ratio of individual phospholipid fractions and of free to ester-bound cholesterol in HDLP determined by computer-aided densitometry of chromatograms coincides with the data obtained routinely. Such a method for assessing the HDLP lipid composition is useful in studies of HDLP in patients with disorders of HDLP metabolism and for assessing the changes in HDLP composition during hypolipidemic therapy.

摘要

采用两种溶剂系统在硅胶薄层上进行一维色谱法分离高密度脂蛋白（HDLP）的脂质提取物，在血脂正常的受试者中检测到10种脂质组分。鉴定出4种磷脂组分：3种主要组分（磷脂酰胆碱、溶血磷脂酰胆碱和鞘磷脂）和1种次要组分磷脂酰乙醇胺。HDLP脂质包括游离胆固醇及其酯；检测到3种甘油酯组分：单甘油酯、二甘油酯和三甘油酯，以及游离脂肪酸。通过色谱图的计算机辅助光密度测定法确定的HDLP中各磷脂组分的比例以及游离胆固醇与酯化胆固醇的比例与常规获得的数据一致。这种评估HDLP脂质组成的方法在HDLP代谢紊乱患者的HDLP研究以及评估降血脂治疗期间HDLP组成的变化方面很有用。