Agafonov V A
Zh Nevropatol Psikhiatr Im S S Korsakova. 1975;75(7):1083-7.
The paper deals with the description of the method which permits to prepare during 3.5 hours the nervous tissue for an electrono-microscopic study. The accelarated processing is achieved due to the reduction of the main stages: prefixation, fixation, dehydration, eponic saturation and polymerization. The isotonic buffer was used for the removal of a possible deformation of cell appendexes (pressing) with the fixation in glutaraldehyde. The accelarated method of the nervous tissue processing neither gives afrtefacts, nor reveals essential differences in the tissue in comparison with the general method.
本文论述了一种能在3.5小时内制备用于电子显微镜研究的神经组织的方法。加速处理是通过减少主要步骤实现的:预固定、固定、脱水、环氧树脂浸透和聚合。使用等渗缓冲液以消除在戊二醛固定过程中细胞附属物可能出现的变形(挤压)。与常规方法相比,这种神经组织加速处理方法既不会产生假象,也不会在组织中显示出本质差异。