Balasenthil S, Rao K S, Nagini S
Department of Biochemistry, Faculty of Science, Annamalai University, Tamil Nadu, India.
Oral Oncol. 2002 Jul;38(5):431-6. doi: 10.1016/s1368-8375(01)00084-7.
The apoptosis-inducing capacity of aqueous garlic extract during 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch (HBP) carcinogenesis was investigated in male Syrian hamsters using DNA fragmentation and the apoptosis-associated proteins, tissue transglutaminase (tTG) and Bcl-2. Hamsters were divided into four groups of six animals each. Animals in group 1 were painted with a 0.5% solution of DMBA in liquid paraffin on the right buccal pouches three times a week for 14 weeks. Group 2 animals painted with DMBA as in group 1, in addition received 250 mg/kg body weight aqueous garlic extract orally on days alternate to DMBA application. Group 3 animals received garlic extract as in group 2. Group 4 animals received neither DMBA nor garlic extract and served as the control. The experiment was terminated at the end of 14 weeks. Administration of aqueous garlic extract (250 mg/kg body weight) to animals painted with DMBA inhibited DMBA-induced oral carcinogenesis as revealed by the absence of neoplasms, induction of tTG and inhibition of Bcl-2 expression. The results of the present study suggest that garlic may exert its chemopreventive effect by inducing apoptosis.
利用DNA片段化以及凋亡相关蛋白、组织转谷氨酰胺酶（tTG）和Bcl-2，在雄性叙利亚仓鼠中研究了水蒜提取物在7,12-二甲基苯并[a]蒽诱导的仓鼠颊囊（HBP）癌变过程中的凋亡诱导能力。仓鼠被分为四组，每组六只动物。第1组动物每周三次在右侧颊囊涂抹0.5%的二甲基苯并蒽（DMBA）液体石蜡溶液，持续14周。第2组动物如第1组那样涂抹DMBA，此外在与涂抹DMBA交替的日子里口服250毫克/千克体重的水蒜提取物。第3组动物如第2组那样接受蒜提取物。第4组动物既不接受DMBA也不接受蒜提取物，作为对照。实验在14周结束时终止。对涂抹DMBA的动物给予水蒜提取物（250毫克/千克体重）可抑制DMBA诱导的口腔癌变，表现为无肿瘤、诱导tTG以及抑制Bcl-2表达。本研究结果表明，大蒜可能通过诱导凋亡发挥其化学预防作用。
