Muninathan N
Department of Research, Meenakshi Medical College and Research Institute, Meenakshi Academy of Higher Education and Research, Enathur, Kanchipuram, 631552 Tamil Nadu India.
Indian J Clin Biochem. 2021 Apr;36(2):143-150. doi: 10.1007/s12291-019-0817-7. Epub 2019 Mar 15.
The purpose of this study was to investigate the Bcl2, P and apoptosis changes against skin cancer in experimental animals. Skin cancer is the most common form of human cancer. It is estimated that over 1 million new cases occur annually. The annual rates of all forms of skin cancer are increasing each year, representing a growing public concern. It has also been estimated that nearly half of all Americans who live to age 65 are likely to develop skin cancer at least once. Skin cancer was induced in rats by Di Methyl Benz (a) Anthracene at the dosage of DMBA (5 µg) per animal, three times a week for 28 weeks after conformation of skin cancer treated with Paclitaxel and Di allyl sulfide for 30 days. The levels of Bcl2 gene expression were significantly decreased and Pgene expression were markedly increased in Paclitaxel and Di allyl sulfide treated animals when compared with cancer bearing animals. The treatment with combination of Paclitaxel and Di allyl sulfide effectively reduced Bcl2 protein expression and also increased Pgene expression. Moreover, the levels of Bcl2 and P a good indicators of restoring the skin architecture, were also reversed in skin damage subjects after treatment with the herbal compounds preparation. So, from the obtained results it is concluded that a combination of Paclitaxel and Di allyl sulfide is capable of restoring the skin architecture and can also increase the apoptosis activities in skin cancer rats.
本研究的目的是调查实验动物中Bcl2、P和凋亡在皮肤癌中的变化。皮肤癌是人类癌症最常见的形式。据估计，每年有超过100万新病例出现。所有形式皮肤癌的年发病率每年都在上升，这引起了公众越来越多的关注。据估计，活到65岁的美国人中近一半可能至少患过一次皮肤癌。在用紫杉醇和二烯丙基硫醚治疗30天确诊皮肤癌后，以每只动物5微克二甲基苯并(a)蒽的剂量，每周三次，连续28周给大鼠诱发皮肤癌。与患癌动物相比，紫杉醇和二烯丙基硫醚治疗的动物中Bcl2基因表达水平显著降低，P基因表达明显增加。紫杉醇和二烯丙基硫醚联合治疗有效降低了Bcl2蛋白表达，同时也增加了P基因表达。此外，Bcl2和P的水平是恢复皮肤结构的良好指标，在用草药化合物制剂治疗后，皮肤损伤受试者的这些指标也得到了逆转。因此，从获得的结果可以得出结论，紫杉醇和二烯丙基硫醚的组合能够恢复皮肤结构，还能增加皮肤癌大鼠的凋亡活性。
