Lundberg Jonas, Lindgård Ann, Elander Anna, Soussi Bassam
Bioenergetics Group, Department of Surgery, Wallenberg Laboratory, Sahlgrenska University Hospital, Göteborg University, 413 45 Göteborg, Sweden.
Microsurgery. 2002;22(4):158-64. doi: 10.1002/micr.21744.
It is of great clinical interest to improve postischemic tissue recovery during microsurgical transfers. The effect of singlet oxygen energy (SOE) as photon illumination at 634 nm on rat skeletal muscle during ischemia and postischemic reperfusion was investigated noninvasively and continuously by in vivo (31)P-magnetic resonance spectroscopy ((31)P-MRS). A model of pedicled rat rectus femoris muscle was used, where phosphorous metabolites were followed before onset of ischemia (control), after 4 h of ischemia, and after 1 h of reperfusion. Two groups were studied: one control group (n = 10), and one SOE-treated group (n = 10). Blood perfusion was measured by laser Doppler flowmetry (LDF) during the study. After 4 h of ischemia, ATP levels were 72% and 51% of normal control values in the illuminated group and the control group, respectively (P < 0.05). After 1 h of postischemic reperfusion, phosphocreatine (PCr) recovered to 79% and adenosine triphosphate (ATP) to 71% in the illuminated group, whereas in the control group, the recovery was 57% and 51%, respectively (P < 0.05). It is concluded that singlet oxygen energy has a beneficial effect on the energy state of skeletal muscle during ischemia and postischemic reperfusion.
在显微外科组织移植过程中改善缺血后组织恢复具有重大临床意义。通过体内（31）P磁共振波谱法（（31）P-MRS）对634nm单态氧能量（SOE）作为光子照射在大鼠骨骼肌缺血及缺血后再灌注期间的作用进行了无创且连续的研究。采用带蒂大鼠股直肌模型，在缺血开始前（对照）、缺血4小时后及再灌注1小时后跟踪磷代谢物。研究了两组：一组为对照组（n = 10），一组为SOE处理组（n = 10）。研究期间通过激光多普勒血流仪（LDF）测量血流灌注。缺血4小时后，光照组和对照组的ATP水平分别为正常对照值的72%和51%（P < 0.05）。缺血后再灌注1小时后，光照组的磷酸肌酸（PCr）恢复到79%，三磷酸腺苷（ATP）恢复到71%，而对照组的恢复率分别为57%和51%（P < 0.05）。结论是单态氧能量在缺血及缺血后再灌注期间对骨骼肌的能量状态具有有益作用。
