Cristoni Simone, Bernardi Luigi Rossi, Biunno Ida, Guidugli Federico
Università degli Studi di Milano, Centro Interdisciplinare Studi Bio-Molecolari Applicazioni Industriali CISI, Via Fratelli Cervi 93, 20090 Segrate, Milano, Italy.
Rapid Commun Mass Spectrom. 2002;16(12):1153-9. doi: 10.1002/rcm.693.
A new approach, based on the use of atmospheric pressure chemical ionization ion trap mass spectrometry (APCI-ITMS), but without a corona discharge, was investigated for application to creating and monitoring protein ions. It must be emphasized that APCI is not usually used in protein analysis. In order to verify the applicability of the proposed method to the analysis of proteins, two standard proteins (horse cytochrome c and horse myoglobin) were analyzed. A mixture of the two proteins was also analyzed showing that this novel approach, based on the use of APCI, can be used in the analysis of protein mixtures.
研究了一种基于大气压化学电离离子阱质谱（APCI-ITMS）但无电晕放电的新方法，用于生成和监测蛋白质离子。必须强调的是，APCI通常不用于蛋白质分析。为了验证所提出的方法对蛋白质分析的适用性，分析了两种标准蛋白质（马细胞色素c和马肌红蛋白）。还分析了这两种蛋白质的混合物，结果表明这种基于APCI的新方法可用于蛋白质混合物的分析。
