Shiino M, Rennels E G
Am J Anat. 1975 Nov;144(3):399-405. doi: 10.1002/aja.1001440314.
Vinblastine sulfate in physiological saline was injected directly into the pituitary glands of lactating rats. Injections were made through the ear canal using a syringe equipped with a 24-gauge needle. The animals were killed at 2, 4, or 6 hours after the injections. When the anterior pituitary glands were examined by electron microscopy, many microtubular paracrystalline deposits were seen in the prolactin and growth hormone cells. The usual cytoplasmic microtubules and microfilaments were not seen in the cytoplasm of these cells. Granular extrusion (exocytosis) was markedly depressed, and an accumulation of secretory granules was definitely observed in the prolactin cells after the administration of vinblastine.
将硫酸长春碱溶于生理盐水中，直接注射到泌乳大鼠的垂体中。使用配备24号针头的注射器通过耳道进行注射。在注射后2、4或6小时处死动物。当通过电子显微镜检查垂体前叶时，在催乳素细胞和生长激素细胞中可见许多微管副晶状沉积物。在这些细胞的细胞质中未见通常的细胞质微管和微丝。颗粒排出（胞吐作用）明显受到抑制，在给予长春碱后，催乳素细胞中确实观察到分泌颗粒的积累。
