Shiino M
Cell Tissue Res. 1980;213(3):433-40. doi: 10.1007/BF00237889.
Unusual paracrystalline aggregates of microtubules which have not been described in any other mammalian species were observed in cultured anterior pituitary cells of normal chinchillas as well as in situ in the pituitary glands of these animals. These aggregates appeared as regularly arranged tubular structures in the longitudinal plane, and as a checkerboard pattern of closely and regularly packed microtubules when examined in transverse section. Supplementation with vinblastine, colcemide or colchicine in the culture medium did not change these structures morphologically. Each unit of tubules consisted of an outer wall or parallelogram profile and an inner wall composed of a single hexagonal doublet or in a figure "8"form. The outer wall of the parallelogram was 35 X 28 nm in length for both sides, while the diagonal of the inner wall was 18 X 28 nm. These paracrystalline aggregates of microtubules in the chinchilla pituitary cells are morphologically distinct from the paracrystalline assembly of cytoplasmic microtubules induced by vinblastine or other alkaloids. The function and significance of these paracrystalline aggregates in anterior pituitary cells of the chinchilla are uncertain.
在正常毛丝鼠培养的垂体前叶细胞以及这些动物垂体的原位组织中，观察到了微管的异常副晶状聚集体，这在其他任何哺乳动物物种中均未被描述过。这些聚集体在纵切面上呈现为规则排列的管状结构，在横切面上则呈现为紧密且规则排列的微管组成的棋盘状图案。在培养基中添加长春碱、秋水仙酰胺或秋水仙素，这些结构在形态上并未改变。每个微管单元由一个外壁或平行四边形轮廓以及一个由单个六边形双联体或以“8”字形组成的内壁构成。平行四边形的外壁两侧长度均为35×28纳米，而内壁的对角线为18×28纳米。毛丝鼠垂体细胞中的这些微管副晶状聚集体在形态上与长春碱或其他生物碱诱导的细胞质微管副晶状组装不同。这些副晶状聚集体在毛丝鼠垂体前叶细胞中的功能和意义尚不确定。
