Maddux William F, Abebe Worku, Schuster George S, Mozaffari Mahmood S
Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia 30912-1128, USA.
J Biomed Mater Res. 2002 Sep 15;61(4):572-80. doi: 10.1002/jbm.10237.
The frequent use of resins in dentistry has raised the question of their compatibility with oral tissues. The present study was undertaken to determine the effects of the resin components methyl methacrylate (MMA), hydroxyethyl methacrylate (HEMA), and triethylene glycol dimethacrylate (TEGDMA) on the reactivity of blood vessels using the isolated rat aorta as a tissue model. MMA, HEMA, and TEGDMA caused endothelium-dependent and -independent relaxation of rat aortic rings in a concentration-related manner. The endothelium-dependent responses of the tissues to all the resins were significantly attenuated by N-nitro-L-arginine methyl ester (L-NAME), indicating the involvement of nitric oxide. The vasorelaxant effects of both MMA and TEGDMA on the intact and denuded aortae were markedly inhibited by indomethacin, providing evidence for the role of prostanoids in these responses. Glybenclamide selectively attenuated TEGDMA-induced relaxation of the tissues with and without endothelium to a similar extent, suggesting the activation of vascular smooth muscle K(ATP) channels by this resin. It is concluded that MMA, HEMA, and TEGDMA interfere with the function of blood vessels by inducing vasorelaxation via different mechanisms, which, depending upon the type of resin, may at least involve the release of nitric oxide and prostanoid(s), and the activation of smooth muscle K(ATP) channels. These phenomena may play a role in tissue homeostasis and certain pathophysiological conditions associated with the application of resin materials to the oral environment.
牙科中树脂的频繁使用引发了其与口腔组织相容性的问题。本研究旨在以离体大鼠主动脉为组织模型，确定树脂成分甲基丙烯酸甲酯（MMA）、甲基丙烯酸羟乙酯（HEMA）和二甲基丙烯酸三甘醇酯（TEGDMA）对血管反应性的影响。MMA、HEMA和TEGDMA以浓度相关的方式引起大鼠主动脉环的内皮依赖性和非内皮依赖性舒张。组织对所有树脂的内皮依赖性反应均被N-硝基-L-精氨酸甲酯（L-NAME）显著减弱，表明一氧化氮参与其中。吲哚美辛显著抑制了MMA和TEGDMA对完整和去内皮主动脉的血管舒张作用，为前列腺素在这些反应中的作用提供了证据。格列本脲选择性地减弱了TEGDMA诱导的有内皮和无内皮组织的舒张，程度相似，表明该树脂激活了血管平滑肌K（ATP）通道。得出的结论是，MMA、HEMA和TEGDMA通过不同机制诱导血管舒张来干扰血管功能，这取决于树脂的类型，可能至少涉及一氧化氮和前列腺素的释放，以及平滑肌K（ATP）通道的激活。这些现象可能在组织稳态以及与在口腔环境中应用树脂材料相关的某些病理生理状况中起作用。
