Abebe Worku, Maddux William F
Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, CL-2130, Medical College of Georgia, Augusta, GA 30912-1128, USA.
Dent Mater. 2006 Jan;22(1):37-44. doi: 10.1016/j.dental.2005.02.008. Epub 2005 Jul 22.
Most dental resinous materials contain the diluent monomer triethyleneglycol dimethacrylate (TEGDMA), which has been reported to be bioactive. Previously, it was demonstrated that TEGDMA induces vasorelaxation. The present study examines the mechanism(s) of the TEGDMA-induced vasorelaxation by measuring vascular nitrite and prostacyclin levels.
Nitrite and prostacyclin levels were assayed in rat aortic tissues in response to TEGDMA. The involvement of guanylyl and adenylyl cyclases in TEGDMA-induced aortic vasorelaxation was determined using the enzyme inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ22536), respectively.
TEGDMA enhanced the levels of nitrites in endothelium-intact and that of protacyclin in both endothelium-intact and denuded rat aortas. The increase in nitrites was associated with endothelium-dependent aortic relaxation mediated via the activation of guanylyl cyclase, while the increase in prostacyclin was associated with both endothelium-dependent and independent relaxation linked to adenylyl cyclase stimulation.
Data from the present investigation can be relevant to dental practice employing materials containing TEGDMA by providing insights into the vasorelaxant effect of the monomer following placement of the materials in the oral cavity. Additional studies that are more relevant to the clinical situation are required to confirm these initial results and further explore their implications.
大多数牙科树脂材料含有稀释单体二甲基丙烯酸三乙二醇酯（TEGDMA），据报道该单体具有生物活性。此前已证实TEGDMA可诱导血管舒张。本研究通过测量血管亚硝酸盐和前列环素水平来探究TEGDMA诱导血管舒张的机制。
检测大鼠主动脉组织对TEGDMA反应时的亚硝酸盐和前列环素水平。分别使用酶抑制剂1H-[1,2,4]恶二唑并[4,3-a]喹喔啉-1-酮（ODQ）和9-(四氢-2-呋喃基)-9H-嘌呤-6-胺（SQ22536）来确定鸟苷酸环化酶和腺苷酸环化酶在TEGDMA诱导的主动脉血管舒张中的作用。
TEGDMA提高了完整内皮大鼠主动脉中亚硝酸盐的水平，以及完整内皮和去内皮大鼠主动脉中前列环素的水平。亚硝酸盐水平的升高与通过鸟苷酸环化酶激活介导的内皮依赖性主动脉舒张有关，而前列环素水平的升高与依赖和不依赖内皮的舒张有关，这与腺苷酸环化酶的刺激有关。
本研究的数据可为口腔中使用含TEGDMA材料的牙科实践提供参考，深入了解材料放置后该单体的血管舒张作用。需要更多与临床情况相关的研究来证实这些初步结果并进一步探讨其意义。
