乳腺癌中流式细胞术检测S期细胞比例：软件和直方图分辨率的影响

Flow cytometric S-phase fraction measurement in breast carcinoma: Influence of software and histogram resolution.

作者信息

Jourdan Marie-Lise, Ferrero-Poüs Magali, Spyratos Frédérique, Romain Sylvie, Martin Pierre-Marie, Chassevent Agnès

机构信息

Laboratoire de Cancérologie, INSERM EMI 0211, Hôpital Bretonneau, CORAD, 2 boulevard Tonnellé, 37044 Tours cedex, France.

出版信息

Cytometry. 2002 Jun 1;48(2):66-70. doi: 10.1002/cyto.10116.

DOI:10.1002/cyto.10116

PMID:12116366

Abstract

BACKGROUND

S-phase fraction (SPF) measurement by flow cytometry is a clinically useful prognostic factor in patients with breast carcinoma. Standardized SPF determination is essential. As part of a multicenter study, we evaluated the influence of the choice of software and histogram resolution (256, 512, or 1,024 channels) on SPF quantification.

METHODS

One hundred thirty-three DNA histograms were analyzed in three laboratories with Modfit 5.2, Modfit LT, and Multicycle AV software. Strict rules for histogram interpretation and software management were applied. The following five options were compared: MF 5.2 1024, MF 5.2 256, MF LT 256, MC AV 256, and MC AV 512.

RESULTS

In the DNA diploid and aneuploid groups, SPF distributions were not statistically different among the five options. Excellent quantitative correlations were obtained between pairs of options. When using tertiles as cutpoints for SPF classification, concordance rates ranged from 79.7% to 93.2% for DNA diploid samples and from 87.8% to 95.9% for DNA aneuploid samples, the best results being obtained with software working with a similar histogram resolution.

CONCLUSIONS

Standardized use of commercially available software, including the choice of histogram resolution, provides comparable SPF results.

摘要

背景

通过流式细胞术测量S期细胞分数（SPF）是乳腺癌患者临床有用的预后因素。标准化的SPF测定至关重要。作为一项多中心研究的一部分，我们评估了软件选择和直方图分辨率（256、512或1024通道）对SPF定量的影响。

方法

在三个实验室中使用Modfit 5.2、Modfit LT和Multicycle AV软件对133个DNA直方图进行分析。应用了严格的直方图解释和软件管理规则。比较了以下五个选项：MF 5.2 1024、MF 5.2 256、MF LT 256、MC AV 256和MC AV 512。

结果

在DNA二倍体和非整倍体组中，五个选项之间的SPF分布无统计学差异。各选项对之间获得了良好的定量相关性。当使用三分位数作为SPF分类的切点时，DNA二倍体样本的一致性率在79.7%至93.2%之间，DNA非整倍体样本的一致性率在87.8%至95.9%之间，使用具有相似直方图分辨率的软件可获得最佳结果。