Zhang Zhuoli, Zhang Guozhu, Dong Yi
Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Beijing 100730, China.
Chin Med J (Engl). 2002 Jun;115(6):856-9.
To explore the pathogenesis of rheumatoid arthritis (RA) by studying the expression of T cell receptors (TCRs).
T cell receptor Vbeta (TCR Vbeta) gene usage and expression were analyzed from synovial membrane and peripheral blood of 8 RA patients, 2 osteoarthritis patients and 2 accident amputees. The complementary determining region 3 (CDR3) of 25 TCR Vbeta subfamily genes in unselected T cell populations were amplified semi-quantitatively by reverse transcription-polymerase chain reaction (RT-PCR). The products were further studied by genescan for frequency of Vbeta usage.
The numbers of Vbeta subfamilies expressed by T cells from RA peripheral blood and synovial membrane were not significantly restricted. More importantly, biased Vbeta gene expression in RA synovium was observed and Vbeta6, Vbeta17, and Vbeta22 genes were the predominant subfamilies. It was noteworthy that the expression of Vbeta17 in RA synovium was significantly increased.
Our data were consistent with the hypothesis that several antigen or superantigen-driven processes may be involved in the pathogenesis of RA.
通过研究T细胞受体（TCRs）的表达来探索类风湿关节炎（RA）的发病机制。
分析了8例RA患者、2例骨关节炎患者和2例意外截肢者的滑膜和外周血中T细胞受体Vβ（TCR Vβ）基因的使用情况和表达。通过逆转录-聚合酶链反应（RT-PCR）半定量扩增未选择的T细胞群体中25个TCR Vβ亚家族基因的互补决定区3（CDR3）。通过基因扫描进一步研究产物以分析Vβ使用频率。
RA外周血和滑膜中T细胞表达的Vβ亚家族数量没有明显受限。更重要的是，在RA滑膜中观察到Vβ基因表达存在偏向性，Vβ6、Vβ17和Vβ22基因是主要的亚家族。值得注意的是，RA滑膜中Vβ17的表达显著增加。
我们的数据与以下假设一致，即几种抗原或超抗原驱动的过程可能参与RA的发病机制。
