Geng Su-xia, Li Yang-qiu, Chen Shao-hua, Yang Li-jian, Yin Qing-song, Wu Xiu-li, Zhang Xue-li
Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China.
Zhonghua Xue Ye Xue Za Zhi. 2005 Jul;26(7):413-6.
To analyze peripheral blood naive T cell level, its T cell receptor (TCR) Vbeta repertoire usage profile and clonality for evaluating the recent thymic output function and the expansion feature of TCR Vbeta subfamily T cells in patients with chronic myelogenous leukemia (CML).
Quantitative detection of T-cell receptor excision DNA circles (TRECs) in peripheral blood mononuclear cells (PBMNC) from 20 cases of CML was preformed by real-time PCR (TaqMan) analysis, and TRECs-number in T-cells was calculated from peripheral blood CD3-positive cell rate. The expression and clonality analysis were detected by RT-PCR and Genescan technique in PBMNC from 14 out of the 20 patients. Nine normal individuals served as controls.
A dramatic reduction of TRECs value in patients with CML was detected as compared with that in normal controls. The mean value of TRECs was 0.06 +/- 0.16 copy/1000 CD3(+) cells in CML patients while 6.84 +/- 4.71 copies/1000 CD3(+) cells in normal controls (P < 0.01). The 1 - 12 Vbeta subfamilies were variably expressed in samples from 14 patients. Genescan analysis identified clonal expanded T cells of some Vbeta subfamily from 13 cases. Vbeta3, Vbeta10, Vbeta19, Vbeta21 and Vbeta22 subfamilies clonal T cells were more frequently seen.
There is a prominent reduction of recent thymic output naive T cells function in CML. The predominant usage and clonal expansion of TCR Vbeta subfamily T cells could be identified, indicating that CML patients have specific immune response to leukemia associated antigen, in spite of their T cell immunodeficiency.
分析慢性髓性白血病（CML）患者外周血初始T细胞水平、T细胞受体（TCR）Vβ谱系使用情况及克隆性，以评估近期胸腺输出功能及TCR Vβ亚家族T细胞的扩增特征。
采用实时荧光定量PCR（TaqMan）分析法对20例CML患者外周血单个核细胞（PBMNC）中的T细胞受体切除环（TRECs）进行定量检测，并根据外周血CD3阳性细胞率计算T细胞中的TRECs数量。对20例患者中的14例患者的PBMNC进行RT-PCR和基因扫描技术检测其表达及克隆性分析。9名正常个体作为对照。
与正常对照相比，CML患者的TRECs值显著降低。CML患者TRECs的平均值为0.06±0.16拷贝/1000个CD3(+)细胞，而正常对照为6.84±4.71拷贝/1000个CD3(+)细胞（P<0.01）。14例患者样本中1-12个Vβ亚家族表达各异。基因扫描分析从13例中鉴定出一些Vβ亚家族的克隆性扩增T细胞。Vβ3、Vβ10、Vβ19、Vβ21和Vβ22亚家族的克隆性T细胞更为常见。
CML患者近期胸腺输出初始T细胞功能显著降低。可识别出TCR Vβ亚家族T细胞的主要使用情况及克隆性扩增，表明CML患者尽管存在T细胞免疫缺陷，但对白血病相关抗原有特异性免疫反应。
