通过组成型表达马立克氏病病毒（MDV）糖蛋白E来产生支持MDV高效生长的永久细胞系。

Generation of a permanent cell line that supports efficient growth of Marek's disease virus (MDV) by constitutive expression of MDV glycoprotein E.

作者信息

Schumacher Daniel, Tischer B Karsten, Teifke Jens-Peter, Wink Kerstin, Osterrieder Nikolaus

机构信息

Institute of Molecular Biology1 and Infectology2, Friedrich-Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, Boddenblick 5a, D-17498 Insel Riems, Germany.

出版信息

J Gen Virol. 2002 Aug;83(Pt 8):1987-1992. doi: 10.1099/0022-1317-83-8-1987.

DOI:10.1099/0022-1317-83-8-1987

PMID:12124462

Abstract

A recombinant cell line (SOgE) was established, which was derived from the permanent quail muscle cell line QM7 and constitutively expressed the glycoprotein E (gE) gene of Marek's disease virus serotype 1 (MDV-1). The SOgE cell line supported growth of virulent (RB-1B) and vaccine (CVI988, 584Ap80C) MDV-1 strains at a level comparable with that of primary chicken embryo cells (CEC). The SOgE cell line was used to produce a vaccine against Marek's disease. Chickens were immunized at 1 day old with 10(3) p.f.u. CVI988 produced on either CEC or SOgE cells. Challenge infection was performed at day 12 with hypervirulent Italian MDV-1 strain EU1. Whereas 7/7 or 6/6 animals, respectively, immunized with SOgE or QM7 cells alone developed Marek's disease, only 1/8 animals from both CVI988-immunized groups exhibited signs of disease, suggesting that SOgE cells are a valuable permanent cell culture system for MDV-1 vaccine production.

摘要

建立了一种重组细胞系（SOgE），其源自鹌鹑永久性肌肉细胞系QM7，并组成型表达1型马立克氏病病毒（MDV-1）的糖蛋白E（gE）基因。SOgE细胞系支持强毒株（RB-1B）和疫苗株（CVI988、584Ap80C）MDV-1的生长，其水平与原代鸡胚细胞（CEC）相当。SOgE细胞系用于生产抗马立克氏病疫苗。1日龄雏鸡用在CEC或SOgE细胞上生产的10³蚀斑形成单位（p.f.u.）CVI988进行免疫。在第12天用超强毒意大利MDV-1毒株EU1进行攻毒感染。单独用SOgE或QM7细胞免疫的动物分别有7/7或6/6发生马立克氏病，而两个CVI988免疫组中只有1/8的动物出现疾病迹象，这表明SOgE细胞是用于生产MDV-1疫苗的有价值的永久性细胞培养系统。