[从豌豆叶片中分离叶绿体和叶绿体DNA]
[Isolation of chloroplasts and chloroplast DNA from pea leaves].
作者信息
Amerkhanova M B, Boguspaev K K, Sliusarenko A G
出版信息
Biokhimiia. 1975 Nov-Dec;40(6):1312-4.
PMID:1212467
Abstract
A method of isolating DNA from pea chloroplasts (ch-DNA) in CsCl density gradient is described. DNA preparations are free of 5-methylcytosine and have a melting temperature of 86.5 degrees. Denatured DNA molecules completely reassociate for 3 hours at 60 degrees C. It is concluded that the preparations obtained are pure ch-DNA.
摘要
描述了一种在氯化铯密度梯度中从豌豆叶绿体分离DNA（ch-DNA）的方法。DNA制品不含5-甲基胞嘧啶，其解链温度为86.5摄氏度。变性的DNA分子在60摄氏度下3小时完全重新结合。得出的结论是，所获得的制品是纯的ch-DNA。
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