[成纤维细胞培养中细胞内游离脯氨酸池的测定]

[Determination of free intracellular proline pool in fibroblast cultures].

作者信息

Chamson A, Perier C, Rattner A, Frey J

出版信息

C R Seances Acad Sci D. 1979 Nov 26;289(14):1049-52.

Abstract

By using a direct, rapid, sensitive colorimetric method, free intracellular proline pool from human adult skin fibroblasts in monolayer was determined. Thus under identical experimental conditions, free intracellular proline pool was about 20 pmol proline/micrograms cellular proteins, whatever the cell lines from human adult skin fibroblasts, the passage and the time of subculture. Besides, there was a poor correlation between collagen biosynthesis in fibroblast cultures and free intracellular proline pool.

摘要

通过使用一种直接、快速、灵敏的比色法，测定了单层培养的成人皮肤成纤维细胞中的细胞内游离脯氨酸池。因此，在相同的实验条件下，无论成人皮肤成纤维细胞系、传代次数和传代培养时间如何，细胞内游离脯氨酸池约为20 pmol脯氨酸/微克细胞蛋白。此外，成纤维细胞培养中的胶原蛋白生物合成与细胞内游离脯氨酸池之间的相关性较差。

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