Chamson A, Ronzière M C, Périer C, Frey J
C R Seances Acad Sci D. 1980 Sep 15;291(2):211-3.
The collagen secreted by dermal fibroblast cell cultures, previously labelled with 3H-proline, was extracted and purified by carboxymethyl cellulose. The ratio of hydroxyproline to proline showed that the collagen produced by cell cultures may be under-hydroxylated in comparison to the ratio observed in the types of collagen extracted from human skin. Then, it is concluded that it is not sufficient to determine only the hydroxyproline content for a collagen biosynthesis study in cell cultures. This low hydroxyproline content may explain the rapid catabolism of a part os newly synthetized collagen in vitro.
用3H-脯氨酸预先标记的真皮成纤维细胞培养物分泌的胶原蛋白，通过羧甲基纤维素进行提取和纯化。羟脯氨酸与脯氨酸的比例表明，与从人类皮肤中提取的胶原蛋白类型中观察到的比例相比，细胞培养物产生的胶原蛋白可能羟化不足。因此得出结论，在细胞培养物的胶原蛋白生物合成研究中，仅测定羟脯氨酸含量是不够的。这种低羟脯氨酸含量可能解释了体外新合成的部分胶原蛋白的快速分解代谢。
