Balboni G C, Brandi M L, Zonefrati L, Repice F
Bull Assoc Anat (Nancy). 1985 Mar;69(204):15-8.
The effects of the low power laser irradiation (Space Mix 5 Mid Laser) on the 3H-proline incorporation in the collagenic proteins produced by two lines of normal human fibroblasts in vitro were studied. The 3H-thymidine incorporation in cultures of control and irradiated fibroblasts of the same lines was also evaluated. The obtained results show that in the experimental conditions considered, laser irradiation may have a positive effect on the production of collagen by the fibroblasts in vitro. This effect is dependent on the time of exposure, the frequency of infra-red impulses and the age of subjects from which fibroblasts were obtained. The laser irradiation in the same experimental conditions does not affect the incorporation of 3H-thymidine in the fibroblasts in vitro. Therefore, the observed increase of collagen production is not dependent on an increase of cellular population.
研究了低功率激光照射（Space Mix 5 Mid Laser）对两株正常人成纤维细胞体外产生的胶原蛋白中3H-脯氨酸掺入的影响。还评估了同一株对照和成纤维细胞照射后的培养物中3H-胸腺嘧啶核苷的掺入情况。所得结果表明，在所考虑的实验条件下，激光照射可能对体外成纤维细胞产生胶原蛋白具有积极作用。这种作用取决于暴露时间、红外脉冲频率以及获取成纤维细胞的受试者年龄。在相同实验条件下，激光照射不会影响体外成纤维细胞中3H-胸腺嘧啶核苷的掺入。因此，观察到的胶原蛋白产量增加并不依赖于细胞数量的增加。
