Ganzera Markus, Zhao Jianping, Muhammad Ilias, Khan Ikhlas Ahmad
National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University 38577, USA.
Chem Pharm Bull (Tokyo). 2002 Jul;50(7):988-91. doi: 10.1248/cpb.50.988.
Lepidium meyenii (Maca) is one of the few plants that can be cultivated in the harsh climate of the Andes. Its nutritious hypocotyl is traditionally used as food and medicine, and Maca products are increasingly becoming popular in the western world as tonics. This paper describes the first analytical method allowing the determination of the main macamides and macaenes, the marker compounds of L. meyenii. A separation within 35 min was possible by using a C-12 stationary phase, an acidic mobile phase comprising of acetonitrile and water, and raising the column temperature to 40 degrees C. By monitoring the separation at 210 and 280 nm, the markers were detectable as low as 0.40 microg/ml. In order to validate the method, accuracy, precision, linearity, limit of detection and intra/inter day repeatability were determined. The analysis of several commercially available Maca products showed a similar qualitative pattern but significant differences in the quantitative composition. The percentage of total markers in the preparations varied from 0.15 to 0.84%, resulting in daily intakes for the consumer from 1.52 to 14.88 mg, respectively.
玛咖是少数能在安第斯山脉恶劣气候条件下种植的植物之一。其营养丰富的下胚轴传统上用作食物和药物，玛咖产品在西方世界作为滋补品越来越受欢迎。本文描述了首个可测定玛咖主要玛卡酰胺和玛卡烯（玛咖的标志性化合物）的分析方法。使用C-12固定相、由乙腈和水组成的酸性流动相并将柱温升至40℃，可在35分钟内实现分离。通过在210和280nm处监测分离情况，标志物的检测限低至0.40微克/毫升。为验证该方法，测定了准确度、精密度、线性、检测限以及日内/日间重复性。对几种市售玛咖产品的分析显示出相似的定性模式，但在定量组成上存在显著差异。制剂中总标志物的百分比从0.15%到0.84%不等，消费者的每日摄入量分别为1.52至14.88毫克。
