Ma Baojin, Xiang Jianbin, Cai Duan, Zhang Yanling, Zha Xiliang, Wang Liying
Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, China.
Zhonghua Wai Ke Za Zhi. 2002 Apr;40(4):298-300.
To evaluate the pro-nucleating activity in 33.5 x 10(3) vesicular protein.
The model biles were established according Kibe and Zhu. The pro-nucleating activity of 33.5 x 10(3) vesicular protein were examined by polarized light microscopy. The protein and its enzymatic deglycosylation and proteolysis fractions nucleation promoting activity were detected by cholesterol crystal growth assay.
33.5 x 10(3) vesicular protein displayed apparent potency of pro-nucleation with activity of 0.310, and derived crystal growth curve indices It, Ig, Ic were presented as 0.57, 1.52, 1.63 respectively, but after treated by N-glycanase enzyme and pronase, no promoting activity were found.
The 33.5 x 10(3) vesicular protein may be involved in the nucleation process of gallstone formation, which is regulated by its peptide and sugar chain.
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