Wang W, Han T, Zhang S
Department of Surgery, Rui Jin Hospital, Shanghai Second Medical University.
Zhonghua Yi Xue Za Zhi. 1995 May;75(5):291-4, 320.
We studied the effect of free fatty acids on cholesterol nucleation in model bile with a sensitive cholesterol crystal growth assay. Palmitic acid, oleic acid, linoleic acid and arachidonic acid were added to model biles in increasing concentrations from 0.5 to 20 umol/ml. The nucleation time of model bile with free fatty acids decreased to 30%-50% of control bile. The final crystal concentration increased with higher concentrations of palmitic acid. However, we found a decrease in the final crystal concentration with the unsaturated fatty acid-soleic acid, linoleic acid and arachidonic acid. The increase in the maximum crystal growth rate was marked in the model biles as linoleic acid was added. These findings show the effect of non-protein fraction in biles on gallstone formation and support the view that free fatty acids are pro-nucleating factors.
我们通过一项灵敏的胆固醇晶体生长试验，研究了游离脂肪酸对模拟胆汁中胆固醇成核的影响。将棕榈酸、油酸、亚油酸和花生四烯酸以0.5至20 μmol/ml的递增浓度添加到模拟胆汁中。含有游离脂肪酸的模拟胆汁的成核时间降至对照胆汁的30%至50%。随着棕榈酸浓度的升高，最终晶体浓度增加。然而，我们发现不饱和脂肪酸（油酸、亚油酸和花生四烯酸）会使最终晶体浓度降低。添加亚油酸时，模拟胆汁中最大晶体生长速率显著增加。这些发现表明胆汁中的非蛋白质成分对胆结石形成的影响，并支持游离脂肪酸是促成核因子这一观点。
