[在模拟胆汁系统中对胆固醇胆结石中鉴定出的细菌的促核活性的研究]
[The study of the pro-nucleating activity of bacteria identified in cholesterol gallstones in model bile systems].
作者信息
Zhu Lei-ming, Cai Duan, Lü Yuan, Chen Wei-huan, Wang Wen-feng, Zhang Yan-ling
机构信息
Department of Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2004 Dec 22;42(24):1501-4.
OBJECTIVE
To explore the relationship of bacteria identified in cholesterol gallstones and gallstone formation.
METHODS
Observe the bacteria activity in model bile and the influence of bacteria on the cholesterol nucleation time (NT).
RESULTS
(1) Model bile were suitable for the growth of E. coli, Pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, clostridium difficile and Clostridium. Propionibacterium acne grew weakly and the growth of Bacteroides fragilis was restrained in model bile. (2) Only pseudomonas aeruginosa and enTerococcus faecalis could ly shorten the cholesterol nucleation time. (3) With pseudomonas aeruginosa or enTerococcus faecalis added in model bile, the formation of cholesterol crystals presented a progressive course of evolution.
CONCLUSIONS
Pseudomonas aeruginosa and enterococcus faecalis, not propionibacterium acne, have pro-nucleating ability in model bile.
目的
探讨胆固醇结石中鉴定出的细菌与胆石形成的关系。
方法
观察模型胆汁中的细菌活性以及细菌对胆固醇成核时间（NT）的影响。
结果
（1）模型胆汁适合大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、粪肠球菌、艰难梭菌和梭菌生长。痤疮丙酸杆菌生长较弱，脆弱拟杆菌在模型胆汁中的生长受到抑制。（2）只有铜绿假单胞菌和粪肠球菌能缩短胆固醇成核时间。（3）在模型胆汁中添加铜绿假单胞菌或粪肠球菌后，胆固醇晶体的形成呈现出一个渐进的演变过程。
结论
在模型胆汁中，具有促成核能力的是铜绿假单胞菌和粪肠球菌，而非痤疮丙酸杆菌。
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