从感染性 cDNA 克隆中恢复强毒和 RNase 阴性减毒 2 型牛病毒性腹泻病毒。
Recovery of virulent and RNase-negative attenuated type 2 bovine viral diarrhea viruses from infectious cDNA clones.
作者信息
Meyer Christiane, Von Freyburg Martina, Elbers Knut, Meyers Gregor
机构信息
Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, D-72001 Tübingen, Germany.
出版信息
J Virol. 2002 Aug;76(16):8494-503. doi: 10.1128/jvi.76.16.8494-8503.2002.
Abstract
Cloned cDNA derived from the genome of the virulent type 2 bovine viral diarrhea virus (BVDV) strain NY'93/C was sequenced and served for establishment of the infectious cDNA clone pKANE40A. Virus recovered from pKANE40A exhibited growth characteristics similar to those of wild-type BVDV NY'93/C and proved to be clinically indistinguishable from the wild-type virus in animal experiments. A virus mutant in which the RNase residing in the viral glycoprotein E(rns) was inactivated, revealed an attenuated phenotype. The plasmid pKANE40A represents the first infectious cDNA clone established for a type 2 BVDV and offers a variety of new approaches to analyze the mechanisms of BVDV-induced disease in cattle.
摘要
对源自强毒2型牛病毒性腹泻病毒（BVDV）NY'93/C株基因组的克隆cDNA进行测序，并用于构建感染性cDNA克隆pKANE40A。从pKANE40A中回收的病毒表现出与野生型BVDV NY'93/C相似的生长特性，并且在动物实验中证明其在临床上与野生型病毒无法区分。一种病毒突变体，其中存在于病毒糖蛋白E(rns)中的核糖核酸酶失活，表现出减毒表型。质粒pKANE40A是为2型BVDV建立的首个感染性cDNA克隆，为分析BVDV诱导牛发病的机制提供了多种新方法。
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本文引用的文献
1
Differences in experimental virulence of bovine viral diarrhoea viral strains isolated from haemorrhagic syndromes.
Vet J. 2000 Nov;160(3):250-8. doi: 10.1053/tvjl.2000.0500.
2
Phylogenetic, antigenic and clinical characterization of type 2 BVDV from North America.
Vet Microbiol. 2000 Nov 15;77(1-2):145-55. doi: 10.1016/s0378-1135(00)00271-6.
3
The genetic basis for cytopathogenicity of pestiviruses.
Vet Microbiol. 2000 Nov 15;77(1-2):117-28. doi: 10.1016/s0378-1135(00)00268-6.
4
E2-p7 region of the bovine viral diarrhea virus polyprotein: processing and functional studies.
J Virol. 2000 Oct;74(20):9498-506. doi: 10.1128/jvi.74.20.9498-9506.2000.
5
A stem-loop motif formed by the immediate 5' terminus of the bovine viral diarrhea virus genome modulates translation as well as replication of the viral RNA.
J Virol. 2000 Jul;74(13):5825-35. doi: 10.1128/jvi.74.13.5825-5835.2000.
6
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7
Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus attenuation.
J Virol. 1999 Dec;73(12):10224-35. doi: 10.1128/JVI.73.12.10224-10235.1999.
8
Genetic diversity of pestiviruses: identification of novel groups and implications for classification.
Virology. 1999 Sep 15;262(1):64-71. doi: 10.1006/viro.1999.9872.
9
Internal initiation of translation of bovine viral diarrhea virus RNA.
Virology. 1999 Jun 5;258(2):249-56. doi: 10.1006/viro.1999.9741.
10
Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles.
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