Becher P, Orlich M, Kosmidou A, König M, Baroth M, Thiel H J
Institut für Virologie (FB Veterinärmedizin), Justus-Liebig-Universität, Giessen, D-35392, Germany.
Virology. 1999 Sep 15;262(1):64-71. doi: 10.1006/viro.1999.9872.
The complete Npro coding sequences were determined for 16 pestiviruses isolated from cattle, pig, and several wild ruminant species including reindeer, bison, deer, and bongo. Phylogenetic analysis enabled the segregation of pestiviruses into the established species bovine viral diarrhea virus-1 (BVDV-1), BVDV-2, border disease virus (BDV), and classical swine fever virus (CSFV). For BVDV-1 five distinct subgroups were identified, while BVDV-2, BDV, and CSFV were each subdivided into two subgroups. The virus isolates from bongo and deer as well as one porcine virus isolate belong to BVDV-1. Interestingly, the isolates from reindeer and bison are distinct from the established pestivirus species. The Npro sequences from these two viruses are more similar to BDV than to the other pestivirus species. Calculation of the pairwise evolutionary distances allowed a clear separation of the categories species, subgroup, and isolate only when the reindeer/bison viruses were considered as members of an additional pestivirus species. Furthermore, the entire E2 coding sequences of a representative set of virus isolates covering all recognized species and subgroups were studied. Segregation of pestiviruses based on the E2 region was identical with that obtained with the N(pro) sequences.
测定了从牛、猪以及包括驯鹿、野牛、鹿和邦戈羚羊在内的几种野生反刍动物物种中分离出的16种瘟病毒的完整Npro编码序列。系统发育分析能够将瘟病毒分为已确定的牛病毒性腹泻病毒1型（BVDV-1）、BVDV-2、边界病病毒（BDV）和经典猪瘟病毒（CSFV）物种。对于BVDV-1，鉴定出了五个不同的亚组，而BVDV-2、BDV和CSFV各自又分为两个亚组。来自邦戈羚羊和鹿的病毒分离株以及一株猪病毒分离株属于BVDV-1。有趣的是，来自驯鹿和野牛的分离株与已确定的瘟病毒物种不同。这两种病毒的Npro序列与BDV的相似性高于与其他瘟病毒物种的相似性。只有当将驯鹿/野牛病毒视为另一种瘟病毒物种的成员时，成对进化距离的计算才能清晰地分离出物种、亚组和分离株类别。此外，还研究了一组覆盖所有公认物种和亚组的代表性病毒分离株的完整E2编码序列。基于E2区域对瘟病毒的分类与用N（pro）序列获得的分类相同。
