Imamura Takahisa, Sugiyama Tatsuo, Cuevas Luis E, Makunde Rachel, Nakamura Shin
Division of Molecular Pathology, Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, Japan.
J Infect Dis. 2002 Aug 1;186(3):436-40. doi: 10.1086/341507. Epub 2002 Jul 5.
The expression of tissue factor (TF), the initiator of the clotting system, was investigated by immunohistochemical staining for its role in clotting mechanisms of Plasmodium falciparum-infected placenta. Most mononuclear cells in the intervillous space of infected placentas stained with an anti-TF monoclonal antibody (MAb) and were positive for antimacrophage MAb. The intervillous space of infected placentas had significant fibrin deposition. In contrast, only small amounts of leukocytes, TF-positive cells, and fibrin were seen in the intervillous space in noninfected placentas. These results indicate that macrophages accumulated in infected placentas express TF and that subsequent perivillous fibrin clot formation leads to a narrowing and plugging of the intervillous space and disturbance of the blood supply. Macrophage TF expression in placentas could be associated with retarded placental growth and low birth weight in malaria infection and should be further investigated.
凝血系统的启动因子组织因子（TF）的表达，通过免疫组织化学染色进行了研究，以探讨其在恶性疟原虫感染胎盘凝血机制中的作用。感染胎盘绒毛间隙中的大多数单核细胞用抗TF单克隆抗体（MAb）染色，且抗巨噬细胞MAb呈阳性。感染胎盘的绒毛间隙有显著的纤维蛋白沉积。相比之下，在未感染胎盘的绒毛间隙中仅可见少量白细胞、TF阳性细胞和纤维蛋白。这些结果表明，积聚在感染胎盘内的巨噬细胞表达TF，随后绒毛周围纤维蛋白凝块的形成导致绒毛间隙变窄和堵塞以及血液供应紊乱。胎盘巨噬细胞TF表达可能与疟疾感染时胎盘生长迟缓及低出生体重有关，应进一步研究。
