正常和超重儿童中血浆肿瘤坏死因子受体1（TNF-R1）及胰岛素浓度与瘦素水平的关系

Plasma TNF-R1 and insulin concentrations in relation to leptin levels among normal and overweight children.

作者信息

Chu Nain Feng, Shen Muh Han, Wu Der Min, Shieh Shyh Ming

机构信息

Department of Community Medicine, Tri-Service General Hospital, National Defense Medical Center, P.R, Taipei, Taiwan, China.

出版信息

Clin Biochem. 2002 Jun;35(4):287-92. doi: 10.1016/s0009-9120(02)00314-4.

DOI:10.1016/s0009-9120(02)00314-4

PMID:12135690

Abstract

OBJECTIVES

Leptin is a multifunctional polypeptide produced primarily by adipocytes and associated with the occurrence of obesity and insulin resistance. Tumor necrosis factor-alpha (TNF-alpha), a polypeptide cytokine produced primarily by mononuclear phagocytes, plays a key role in the initiation of the inflammatory response but has a multitude of effects in many tissues. The interactions between TNF-alpha and insulin on leptin expression are complicated and have not been completely described. The purpose of this study is to evaluate interactions between TNF-alpha and insulin on circulating leptin levels among normal and overweight children.

DESIGN AND METHODS

After multi-stage sampling, we randomly selected 1,500 school children in this survey. All children completed a questionnaire on their disease history and lifestyle characteristics. We measured body weight, height, waist and hip circumference of children and calculated BMI and Waist-to-hip ratio (WHR). We also measured plasma tumor necrosis factor-receptor 1 (TNF-R1) by ELISA, plasma insulin and circulating leptin levels by RIA using commercial kits. We divided the children into normal or overweight groups using BMI criteria (85th percentile) to evaluate the relationship of TNF-R1 and insulin on circulating leptin concentrations.

RESULTS

In general, boys were taller, heavier and had higher TNF-R1 and lower leptin levels than girls. Plasma leptin levels were positively correlated with anthropometric variables and insulin levels among children in both genders. In multivariate regression analyses, plasma insulin levels were significantly positive associated with leptin levels in normal weight children. Plasma TNF-R1 levels were positively associated with leptin levels even after adjusting for BMI in girls only.

CONCLUSIONS

From this study, we found that plasma insulin plays certain role in leptin expression among normal weight children. However, TNF-R1 plays a more significant role in leptin expression among girls only.

摘要

目的

瘦素是一种主要由脂肪细胞产生的多功能多肽，与肥胖症和胰岛素抵抗的发生有关。肿瘤坏死因子-α（TNF-α）是一种主要由单核吞噬细胞产生的多肽细胞因子，在炎症反应的启动中起关键作用，但在许多组织中具有多种效应。TNF-α与胰岛素对瘦素表达的相互作用很复杂，尚未完全阐明。本研究的目的是评估TNF-α与胰岛素对正常和超重儿童循环瘦素水平的相互作用。

设计与方法

经过多阶段抽样，我们在本次调查中随机选择了1500名学童。所有儿童都完成了一份关于他们病史和生活方式特征的问卷。我们测量了儿童的体重、身高、腰围和臀围，并计算了体重指数（BMI）和腰臀比（WHR）。我们还使用商业试剂盒通过酶联免疫吸附测定法（ELISA）测量血浆肿瘤坏死因子受体1（TNF-R1），通过放射免疫分析法（RIA）测量血浆胰岛素和循环瘦素水平。我们使用BMI标准（第85百分位数）将儿童分为正常或超重组，以评估TNF-R1和胰岛素与循环瘦素浓度的关系。