Olszanecka-Glinianowicz Magdalena, Zahorska-Markiewicz Barbara, Janowska Joanna, Zurakowski Aleksander
Department of Pathophysiology, Silesian University School of Medicine, Katowice, Poland.
Metabolism. 2004 Oct;53(10):1268-73. doi: 10.1016/j.metabol.2004.07.001.
The aims of the present study was to examine how overweight and obesity affect serum concentrations nitric oxide (NO) metabolites and to determine whether there is association between serum concentrations tumor necrosis factor (TNF)-alpha and TNF soluble receptors (sTNF-R) in subjects with overweight and obesity. The study groups involved 154 women: 102 obese (81 obese with body mass index [BMI] 30 to 40 kg/m2 and 21 obese with BMI > 40 kg/m2), 24 overweight patients, and 28 lean controls. Serum concentrations of NO metabolites and of TNF-alpha and its soluble receptors (sTNF-R1, sTNFR-2) were measured by enzyme-linked immunosorbent assay (ELISA) kits. Serum concentration of insulin was measured by radioimmunoassay (RIA). Plasma glucose, cholesterol, high-density lipoprotein (HDL)-cholesterol, and triglicerydes were determined by enzymatic procedure. Body composition was determined by impedance analysis using Bodystat (Douglas, British Isles). Serum concentrations of NO in the overweight group (35.1 +/- 12.1 micromol/L) and the obese groups with BMI 30 to 40 kg/m2 (32.8 +/- 9.3 micromol/L) and with BMI greater than 40 kg/m2 (33.3 +/- 8.5 micromol/L) were significantly higher when compared to controls (28.2 +/- 8.1 micromol/L): P < .05; P < .01, and P < .01, respectively. There was no difference in levels of NO between the overweight group and both obese groups. Serum concentration of TNF-alpha was also significantly higher in the group with overweight (6.5 +/- 3.1 pg/mL), in the obese group with BMI 30 to 40 kg/m2 (6.8 +/- 3.1 pg/mL), and in the obese group with BMI greater than 40 kg/m2 (7.4 +/- 2.6 pg/mL) when compared to controls (2.9 +/- 2.2 pg/mL): P < .00005; P < .00005, and P < .0000001, respectively. However, serum concentrations of sTNF-R1 and -R2 did not differ significantly between the overweight group, both obese groups, and controls. In conclusion, we observed increased serum concentrations of TNF-alpha and NO in overweight and obese women. It seems that there is an association between serum concentrations of TNF-alpha and NO; however, this relationship depends on the degree of obesity.
本研究的目的是探讨超重和肥胖如何影响血清一氧化氮（NO）代谢产物的浓度，并确定超重和肥胖受试者血清肿瘤坏死因子（TNF）-α浓度与TNF可溶性受体（sTNF-R）之间是否存在关联。研究组包括154名女性：102名肥胖者（81名体重指数[BMI]为30至40kg/m²的肥胖者和21名BMI>40kg/m²的肥胖者）、24名超重患者和28名瘦对照者。采用酶联免疫吸附测定（ELISA）试剂盒测定血清NO代谢产物、TNF-α及其可溶性受体（sTNF-R1、sTNFR-2）的浓度。采用放射免疫测定（RIA）法测定血清胰岛素浓度。通过酶法测定血浆葡萄糖、胆固醇、高密度脂蛋白（HDL）-胆固醇和甘油三酯。使用Bodystat（道格拉斯，不列颠群岛）通过阻抗分析确定身体成分。与对照组（28.2±8.1μmol/L）相比，超重组（35.1±12.1μmol/L）、BMI为30至40kg/m²的肥胖组（32.8±9.3μmol/L）和BMI大于40kg/m²的肥胖组（33.3±8.5μmol/L）的血清NO浓度显著更高：P<.05；P<.01和P<.01。超重组与两个肥胖组之间的NO水平无差异。与对照组（2.9±2.2pg/mL）相比，超重组（6.5±3.1pg/mL）、BMI为30至40kg/m²的肥胖组（6.8±3.1pg/mL）和BMI大于40kg/m²的肥胖组（7.4±2.6pg/mL）的血清TNF-α浓度也显著更高：P<.00005；P<.00005和P<.0000001。然而，超重组、两个肥胖组和对照组之间的sTNF-R1和-R2血清浓度无显著差异。总之，我们观察到超重和肥胖女性的血清TNF-α和NO浓度升高。似乎血清TNF-α浓度与NO之间存在关联；然而，这种关系取决于肥胖程度。
