利用cDNA微阵列技术发现和分析肝细胞癌基因
Discovery and analysis of hepatocellular carcinoma genes using cDNA microarrays.
作者信息
Li Yao, Li Yali, Tang Rong, Xu Hong, Qiu Minyan, Chen Qin, Chen Juxiang, Fu Zhiren, Ying Kang, Xie Yi, Mao Yumin
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2002 Jul;128(7):369-79. doi: 10.1007/s00432-002-0347-0. Epub 2002 Jun 20.
PURPOSE
Microarray analysis on a genomic scale was used to profile changes in gene expression accompanying hepatocellular carcinoma.
METHODS
Gene expression profiles of liver tissues from twelve hepatocellular carcinoma samples relative to the gene expression profile of the normal liver tissue were analyzed using 4096 chips and 12800 chips. The results of microarray experiments were verified by the Northern blot technique.
RESULTS
A group of 1,820 genes with altered expression were identified in more than 50% of the patients examined. This highly concordant expression profile included human genes encoding proteins involved in the function of peroxisomes, serum control, polycyclic aromatic hydrocarbon carcinogenesis, cell growth and differentiation, metastasis, the function of the immune system, apoptosis, and remodeling of the cytoskeleton.
CONCLUSIONS
The newly identified genes afford a quantitative view of the changes that accompany liver cancer at the genomic level, enable deeper insights into the molecular basis of disease, and provide an extensive list of potential early-onset molecular markers for improved diagnosis.
目的
采用基因组规模的微阵列分析来描绘肝细胞癌伴发的基因表达变化。
方法
使用4096芯片和12800芯片分析了12个肝细胞癌样本的肝组织基因表达谱相对于正常肝组织基因表达谱的情况。微阵列实验结果通过Northern印迹技术进行验证。
结果
在超过50%的受检患者中鉴定出一组1820个表达改变的基因。这种高度一致的表达谱包括编码参与过氧化物酶体功能、血清调控、多环芳烃致癌作用、细胞生长与分化、转移、免疫系统功能、细胞凋亡以及细胞骨架重塑的蛋白质的人类基因。
结论
新鉴定出的基因提供了在基因组水平上伴随肝癌发生的变化的定量视图，能够更深入地洞察疾病的分子基础，并提供大量潜在的早期发病分子标志物清单以改善诊断。
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