Levedakou Eleni N, Freeman David A, Budzynski Michael J, Early Buddy E, Damaso Ruth C, Pollard Anne M, Townley Amy Jo, Gombos Jennifer L, Lewis Jennifer L, Kist Frank G, Hockensmith Mary E, Terwilliger Michelle L, Amiott Elizabeth, McElfresh Kevin C, Schumm James W, Ulery Suzanna R, Konotop Felipe, Sessa Tara L, Sailus Jeffrey S, Crouse Cecelia A, Tomsey Christine S, Ban Jeffrey D, Nelson Mark S
North Carolina State Bureau of Investigation, Raleigh 27603, USA.
J Forensic Sci. 2002 Jul;47(4):757-72.
In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, the GenePrint PowerPlex 2.1 system (PowerPlex 2.1) developed by Promega Corporation and a separate pentanucleotide-repeat locus, Penta D, were tested. This megaplex system includes the highly polymorphic loci FGA, TPOX, D8S1179, vWA, Penta E, D18S51, D21S11, TH01, and D3S1358 and may be used in combination with the eight-locus STR multiplex, the GenePrint PowerPlex 1.1 system (PowerPlex 1.1) that has been previously developed. Three of the loci, TPOX, TH01 and vWA, have been included in both systems for quality control purposes. As with PowerPlex 1.1, PowerPlex 2.1 is also based on a two-color detection of fluorescent-labeled DNA products amplified by polymerase chain reaction (PCR) and provides a valuable tool for accurate and rapid allele determination. The primer sequences used in the PowerPlex 2.1/Penta D system are also presented in this report. To meet the "Quality Assurance Standards for Forensic DNA Testing Laboratories" (FBI), we tested the efficiency and reproducibility of the PowerPlex 2.1/PentaD system by several validation studies that were conducted as a joint project among seven laboratories. Validation tests included concordance studies, sensitivity, and species specificity determination, as well as performance in forensic and environmentally impacted samples. The results produced from these tests demonstrated the consistency and reliability of the PowerPlex 2.1/Penta D system.
为了提高用于人类身份识别的鉴别能力，对由普洛麦格公司开发的一个九基因座短串联重复序列（STR）复合扩增体系——基因打印PowerPlex 2.1系统（PowerPlex 2.1）以及一个单独的五核苷酸重复基因座Penta D进行了测试。这个超级复合扩增体系包括高度多态性基因座FGA、TPOX、D8S1179、vWA、Penta E、D18S51、D21S11、TH01和D3S1358，并且可以与先前开发的八基因座STR复合扩增体系——基因打印PowerPlex 1.1系统（PowerPlex 1.1）联合使用。为了质量控制目的，两个系统都包含了三个基因座TPOX、TH01和vWA。与PowerPlex 1.1一样，PowerPlex 2.1也是基于对通过聚合酶链反应（PCR）扩增的荧光标记DNA产物进行双色检测，并为准确快速的等位基因确定提供了一个有价值的工具。本报告还列出了PowerPlex 2.1/Penta D系统中使用的引物序列。为了符合“法医DNA检测实验室质量保证标准”（联邦调查局），我们通过七个实验室联合开展的多项验证研究，测试了PowerPlex 2.1/PentaD系统的效率和可重复性。验证测试包括一致性研究、灵敏度和物种特异性测定，以及在法医样本和受环境影响样本中的性能。这些测试产生的结果证明了PowerPlex 2.1/Penta D系统的一致性和可靠性。
