Kwon Ji-Hoon, Ahn Young-Joon
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, Republic of Korea.
J Agric Food Chem. 2002 Jul 31;50(16):4479-83. doi: 10.1021/jf020293a.
The acaricidal activity of materials derived from the rhizome of Cnidium officinale against adults of Dermatophagoides farinae and Dermatophagoides pteronyssinus was examined using direct contact application and fumigation methods and compared with that of benzyl benzoate and N,N-diethyl-m-toluamide (DEET). The active constituent of the Cnidium rhizome was identified as butylidenephthalide by spectroscopic analyses. Responses varied with dose. On the basis of 24-h LD(50) values, the acaricidal activity of butylidenephthalide (6.77 microg/cm(2)) against D. farinae adults was comparable to that of benzyl benzoate (8.54 microg/cm(2)). Very low activity was observed with DEET (37.59 microg/cm(2)). Against D. pteronyssinus adults, butylidenephthalide (6.46 microg/cm(2)) and benzyl benzoate (6.68 microg/cm(2)) were equitoxic. DEET (17.98 microg/cm(2)) was relatively inactive. The typical poisoning symptom of butylidenephthalide was lethargy of treated mites, leading to death without knockdown, whereas benzyl benzoate and DEET caused death following uncoordinated behavior. In a fumigation test with both mite species, butylidenephthalide was much more effective in closed containers than open ones. Naturally occurring C. officinale rhizome-derived materials merit further study as potential house dust mite control agents or lead compounds.
采用直接接触法和熏蒸法，检测了蛇床子根茎提取物对粉尘螨和屋尘螨成虫的杀螨活性，并与苯甲酸苄酯和N,N - 二乙基 - 间甲苯甲酰胺（避蚊胺）进行了比较。通过光谱分析确定蛇床子根茎的活性成分是丁烯基苯酞。反应随剂量而变化。根据24小时半数致死剂量（LD50）值，丁烯基苯酞（6.77微克/平方厘米）对粉尘螨成虫的杀螨活性与苯甲酸苄酯（8.54微克/平方厘米）相当。避蚊胺（37.59微克/平方厘米）的活性很低。对于屋尘螨成虫，丁烯基苯酞（6.46微克/平方厘米）和苯甲酸苄酯（6.68微克/平方厘米）的毒性相当。避蚊胺（17.98微克/平方厘米）相对无活性。丁烯基苯酞的典型中毒症状是处理过的螨虫嗜睡，导致死亡而无击倒现象，而苯甲酸苄酯和避蚊胺则导致不协调行为后死亡。在对两种螨虫的熏蒸试验中，丁烯基苯酞在密闭容器中比在开放容器中更有效。天然来源的蛇床子根茎提取物作为潜在的室内尘螨控制剂或先导化合物值得进一步研究。
