Liu W M, Duan E K, Cao Y J
State Key Laboratory of Reproductive Biology Institute of Zoology, Academia Sinica, 19 Zhongguancun Lu, Haidian, Beijing 100080, People's Republic of China.
Life Sci. 2002 Aug 23;71(14):1623-32. doi: 10.1016/s0024-3205(02)01928-8.
Anandamide (N-arachidonoylethanolamine), an arachidonic acid derivative, is an endogenous ligand for both the brain-type (CB1-R) and spleen-type (CB2-R) cannabinoid receptors. To investigate the possible effects of anandamide on embryo implantation in the mouse, we used a co-culture system in which mouse embryos are cultured with a monolayer of uterine epithelial cells. Our results indicate that 14 nM anandamide significantly promotes the attachment and outgrowth of the blastocysts on the monolayer of uterine epithelial cells, and those effects could be blocked by CB1-R antagonists SR141716A, but not by SR144528, a CB2-R antagonist. It suggests that the effects of anandamide on embryo attachment and outgrowth are mediated by CB1-R. However, 56 nM anandamide is capable of inhibiting the blastocyst attachment and outgrowth, we, therefore, conclude that anandamide may play an essential role at the outset of implantation.
花生四烯酸乙醇胺（N-花生四烯酰乙醇胺）是一种花生四烯酸衍生物，是脑型（CB1-R）和脾型（CB2-R）大麻素受体的内源性配体。为了研究花生四烯酸乙醇胺对小鼠胚胎着床的可能影响，我们使用了一种共培养系统，即将小鼠胚胎与单层子宫上皮细胞一起培养。我们的结果表明，14 nM的花生四烯酸乙醇胺显著促进了胚泡在单层子宫上皮细胞上的附着和生长，且这些作用可被CB1-R拮抗剂SR141716A阻断，但不能被CB2-R拮抗剂SR144528阻断。这表明花生四烯酸乙醇胺对胚胎附着和生长的作用是由CB1-R介导的。然而，56 nM的花生四烯酸乙醇胺能够抑制胚泡的附着和生长，因此，我们得出结论，花生四烯酸乙醇胺可能在着床开始时起重要作用。
