Andreopoulos Dimitrios, Kasi LeelapP, Edmund Kim E, Diaz Miguel, Yang David J, Asimacopoulos Panayiotis J
University of Texas M.D. Anderson Cancer Center, Houston, Texas.; From the Baylor College of Medicine, the Methodist, Houston, Texas 77030-4009, USA.
Invest Radiol. 2002 Aug;37(8):435-9. doi: 10.1097/00004424-200208000-00004.
To evaluate the usefulness of size-modified (99m)Tc-labeled liposomes for the detection of acute postoperative mediastinitis in a mouse model.
Fourteen mice underwent low-neck collar incision and had sterile abscesses induced in mediastinum with turpentine. Ten of these animals were injected intravenously with anionic liposomes of 516 +/- 20 nm containing poly(ethylene)glycol labeled with (99m)Tc; the remaining four mice were injected with (67)Ga citrate and used as positive controls. In addition, eight mice either underwent the same surgical procedure but without turpentine (n = 4) or were not operated (n = 4). These were used as negative controls. Therefore, scintigraphy using (99m)Tc-liposomes was performed in eighteen and (67)Ga citrate in four mice. Target area of interest was outlined, and target to background count density ratio and percentage-injected dose were measured.
Significant accumulation of radiolabeled liposomes was observed at sites of inflammation within 1 hour in abscess-bearing animals. This correlated well with the findings of the lower quality (contrast) of (67)Ga images at 24 and 48 hours. The radiopharmaceutical did not significantly accumulate in the mediastinum of negative control animals.
(99m)Tc-liposomes (size modified) may prove useful nonspecific agent for the early diagnosis of postoperative mediastinitis.
评估尺寸改良的（99m）Tc标记脂质体在小鼠模型中检测急性术后纵隔炎的效用。
14只小鼠接受低颈部领口切口，并在纵隔内用松节油诱导无菌脓肿形成。其中10只动物静脉注射含有（99m）Tc标记的聚乙二醇的516±20nm阴离子脂质体；其余4只小鼠注射枸橼酸（67）Ga并用作阳性对照。此外，8只小鼠要么接受相同的手术操作但不使用松节油（n = 4），要么未接受手术（n = 4）。这些用作阴性对照。因此，对18只小鼠进行了使用（99m）Tc脂质体的闪烁显像，对4只小鼠进行了枸橼酸（67）Ga显像。勾勒出感兴趣的目标区域，并测量目标与背景计数密度比以及注射剂量百分比。
在有脓肿的动物中，1小时内炎症部位观察到放射性标记脂质体的显著积聚。这与24小时和48小时时（67）Ga图像较低质量（对比度）的结果密切相关。放射性药物在阴性对照动物的纵隔中未显著积聚。
（99m）Tc脂质体（尺寸改良）可能被证明是用于术后纵隔炎早期诊断的有用非特异性试剂。
