[甲基苯丙胺给药后大鼠体内细胞色素P450 2E1 mRNA表达增加]

[Increase expression of CYP2E1 mRNA after methamphetamine administration in rats].

作者信息

Takimoto Takahiro, Ujike Hiroshi, Nakamura Kazuhiko, Iwahashi Kazuhiko

机构信息

Division of Neurophysiology, Graduate School of Azabu University, 1-17-7 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.

出版信息

Nihon Arukoru Yakubutsu Igakkai Zasshi. 2002 Jun;37(3):163-7.

PMID:12138722

Abstract

Methamphetamine (METH) is one of the most commonly abused psychostimulant. We have investigated the effect of METH administration on the CYP2E1 mRNA level using quantitative competitive reverse transcription-polymerase chain reaction assay. Rats were given METH (4 mg/kg or 8 mg/kg/day) for 2 weeks. The expression of CYP2E1 mRNA increased (5.7 fold) in 4 mg/kg/day administration rat liver in compared with control rat liver. The results suggest that exposure to METH may increase CYP2E1 level but superfluous exposure (8 mg/kg/day) of METH may reduce the expression of CYP2E1 gene.

摘要

甲基苯丙胺（METH）是最常被滥用的精神兴奋剂之一。我们使用定量竞争性逆转录-聚合酶链反应分析方法，研究了给予METH对细胞色素P450 2E1（CYP2E1）mRNA水平的影响。给大鼠连续2周给予METH（4毫克/千克或8毫克/千克/天）。与对照大鼠肝脏相比，给予4毫克/千克/天METH的大鼠肝脏中CYP2E1 mRNA的表达增加（5.7倍）。结果表明，接触METH可能会增加CYP2E1水平，但过量接触（8毫克/千克/天）METH可能会降低CYP2E1基因的表达。