Manuelidis L, Manuelidis E E, Prichard J
J Cell Physiol. 1975 Dec;87(2):179-87. doi: 10.1002/jcp.1040870206.
Cells from a human glioblastoma (TC 526) maintained in tissue culture for ten years had a mean membrane potential of 27 +/- 0.9 mV at an external potassium concentration [Ko] of 5.3 mM. When [Ko] was varied between 2.5 and 5.3 mM, membrane potential changes were close to those predicted by the Nernst equation. At higher [Ko], the Nernstian slope was approached only in the presence of 10(-5) M ouabain, which did not affect membrane potential at a [Ko] of 5.3 mM. An electrogenic sodium pump activated by high [Ko] could explain these findings; such a mechanism has been demonstrated in other tissues.
在组织培养中维持了十年的人胶质母细胞瘤（TC 526）细胞，在细胞外钾浓度[Ko]为5.3 mM时，平均膜电位为27±0.9 mV。当[Ko]在2.5至5.3 mM之间变化时，膜电位变化接近能斯特方程预测的值。在较高的[Ko]时，仅在存在10^(-5) M哇巴因的情况下接近能斯特斜率，而哇巴因在[Ko]为5.3 mM时不影响膜电位。由高[Ko]激活的生电钠泵可以解释这些发现；这种机制已在其他组织中得到证实。
