来自三角涡虫（Girardia tigrina）的一类新的C型凝集素样基因家族的鉴定与表征。

Identification and characterization of a new family of C-type lectin-like genes from planaria Girardia tigrina.

作者信息

Shagin Dmitry A, Barsova Ekaterina V, Bogdanova Ekaterina, Britanova Olga V, Gurskaya Nadia, Lukyanov Konstantin A, Matz Mikhail V, Punkova Natalia I, Usman Natalia Y, Kopantzev Eugene P, Salo Emili, Lukyanov Sergey A

机构信息

Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, 117871 Moscow, Russia.

出版信息

Glycobiology. 2002 Aug;12(8):463-72. doi: 10.1093/glycob/cwf056.

DOI:10.1093/glycob/cwf056

PMID:12145187

Abstract

A novel family of C-type lectin-like genes, denoted multidomain free lectin (MDFL), was identified in the freshwater planaria Girardia (Dugesia) tigrina. We cloned several genes that encode proteins comprising a signal peptide and a number of consecutive C-type lectin-like domains (CTLDs) interconnected by short linker stretches. Analyses of genomic organization, CTLD amino acid sequences, and the overall architecture of these proteins indicate that planarian proteins are a separate family of C-type lectin-like proteins. These genes are expressed in specifically differentiated gland cells of planaria and the corresponding proteins are excreted as components of the planarian body surface mucus.

摘要

在淡水涡虫虎纹三角涡虫（Girardia (Dugesia) tigrina）中鉴定出了一个新的C型凝集素样基因家族，称为多结构域游离凝集素（MDFL）。我们克隆了几个基因，这些基因编码的蛋白质包含一个信号肽和多个由短连接序列相互连接的连续C型凝集素样结构域（CTLD）。对基因组结构、CTLD氨基酸序列以及这些蛋白质的整体结构的分析表明，涡虫蛋白质是C型凝集素样蛋白质的一个独立家族。这些基因在涡虫特定分化的腺细胞中表达，相应的蛋白质作为涡虫体表黏液的成分被分泌出来。