Sakuma Tsutomu, Endo Yusuke, Mashino Misae, Kuroiwa Masami, Ohara Ayako, Jarukamjorn Kanokwan, Nemoto Nobuo
Department of Toxicology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Arch Biochem Biophys. 2002 Aug 15;404(2):234-42. doi: 10.1016/s0003-9861(02)00329-6.
A second female-predominant murine CYP3A, CYP3A44, was isolated from liver and its mRNA expression was compared with that of the previously described CYP3A41. The expression of CYP3A44 was relatively constant after birth in females, whereas it gradually declined in males after 5 weeks of age. The expression of CYP3A41 increased with age in females after 3 weeks of age, whereas it gradually declined in males after 5 weeks of age. Hypophysectomy and growth hormone replacement indicated that expression of both CYP3A mRNAs in females was dependent on the feminine plasma growth hormone profile. Estradiol induced the expression of both mRNAs and the effect was dependent on the presence of the pituitary gland. These observations suggest that endocrine control of expression might be similar, but not identical, for two female-predominant CYP3A mRNAs.
从肝脏中分离出第二种雌性占优势的小鼠CYP3A——CYP3A44，并将其mRNA表达与先前描述的CYP3A41的mRNA表达进行比较。CYP3A44的表达在雌性出生后相对稳定，而在雄性5周龄后逐渐下降。CYP3A41的表达在雌性3周龄后随年龄增加，而在雄性5周龄后逐渐下降。垂体切除和生长激素替代表明，雌性中两种CYP3A mRNA的表达均依赖于雌性血浆生长激素水平。雌二醇诱导了两种mRNA的表达，且该效应依赖于垂体的存在。这些观察结果表明，两种雌性占优势的CYP3A mRNA的表达的内分泌调控可能相似但不完全相同。
