无机多聚磷酸盐在大肠杆菌和聚球藻属PCC6803菌株的phoU突变体中的积累。
Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp. strain PCC6803.
作者信息
Morohoshi Tomohiro, Maruo Tatsuya, Shirai Yoko, Kato Junichi, Ikeda Tsukasa, Takiguchi Noboru, Ohtake Hisao, Kuroda Akio
机构信息
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8530, Japan.
出版信息
Appl Environ Microbiol. 2002 Aug;68(8):4107-10. doi: 10.1128/AEM.68.8.4107-4110.2002.
Abstract
The biological process for phosphate (P(i)) removal is based on the use of bacteria capable of accumulating inorganic polyphosphate (polyP). We obtained Escherichia coli mutants which accumulate a large amount of polyP. The polyP accumulation in these mutants was ascribed to a mutation of the phoU gene that encodes a negative regulator of the P(i) regulon. Insertional inactivation of the phoU gene also elevated the intracellular level of polyP in Synechocystis sp. strain PCC6803. The mutant could remove fourfold more P(i) from the medium than the wild-type strain removed.
摘要
去除磷酸盐（P(i)）的生物学过程基于使用能够积累无机多聚磷酸盐（polyP）的细菌。我们获得了能积累大量多聚磷酸盐的大肠杆菌突变体。这些突变体中多聚磷酸盐的积累归因于编码P(i)调节子负调控因子的phoU基因突变。phoU基因的插入失活也提高了集胞藻PCC6803菌株细胞内多聚磷酸盐的水平。该突变体从培养基中去除的P(i)比野生型菌株多四倍。
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本文引用的文献
1
The Yersinia enterocolitica motility master regulatory operon, flhDC, is required for flagellin production, swimming motility, and swarming motility.
J Bacteriol. 1999 May;181(9):2823-33. doi: 10.1128/JB.181.9.2823-2833.1999.
2
Counterselectable markers: untapped tools for bacterial genetics and pathogenesis.
Infect Immun. 1998 Sep;66(9):4011-7. doi: 10.1128/IAI.66.9.4011-4017.1998.
3
Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response.
J Bacteriol. 1998 Apr;180(8):2186-93. doi: 10.1128/JB.180.8.2186-2193.1998.
4
Novel assay reveals multiple pathways regulating stress-induced accumulations of inorganic polyphosphate in Escherichia coli.
J Bacteriol. 1998 Apr;180(7):1841-7. doi: 10.1128/JB.180.7.1841-1847.1998.
5
Guanosine tetra- and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coli.
J Biol Chem. 1997 Aug 22;272(34):21240-3. doi: 10.1074/jbc.272.34.21240.
6
Molecular analysis of the phosphate-specific transport (pst) operon of Pseudomonas aeruginosa.
Mol Gen Genet. 1996 Apr 10;250(6):692-8. doi: 10.1007/BF02172980.
7
Gene regulation by phosphate in enteric bacteria.
J Cell Biochem. 1993 Jan;51(1):47-54. doi: 10.1002/jcb.240510110.
8
Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.
Appl Environ Microbiol. 1993 Nov;59(11):3744-9. doi: 10.1128/aem.59.11.3744-3749.1993.
9
Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon.
J Bacteriol. 1993 Nov;175(21):6797-809. doi: 10.1128/jb.175.21.6797-6809.1993.
10
Inorganic polyphosphate: toward making a forgotten polymer unforgettable.
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