一种纯化网织红细胞珠蛋白信使核糖核酸的简单方法。
A simple method for the purification of reticulocyte globin messenger ribonucleic acid.
作者信息
Konecki D, Cimadevilla J M, Kramer G, Hardesty B
出版信息
Mol Biol Rep. 1975 Dec;2(4):355-41. doi: 10.1007/BF00357021.
PMID:1214785
Abstract
A relatively simple and inexpensive method has been developed for the preparation of highly purified rabbit reticulocyte globin mRNA. After phenol extraction, polysomal RNA was chromatographed on Sigmacell type 38 cellulose and Sepharose 4B. The resulting mRNA preparation has a purity in excess of 90%. No selective loss of either alpha or beta globin mRNA is observed.
摘要
已开发出一种相对简单且成本低廉的方法来制备高度纯化的兔网织红细胞珠蛋白mRNA。经苯酚抽提后，多聚核糖体RNA在SigmaCell 38型纤维素和琼脂糖4B上进行层析。所得的mRNA制剂纯度超过90%。未观察到α或β珠蛋白mRNA有选择性丢失。
Zotero 插件