控制刚地弓形虫急性毒力的数量性状基因座的鉴定。
Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii.
作者信息
Su Chunlei, Howe Daniel K, Dubey J P, Ajioka James W, Sibley L David
机构信息
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
出版信息
Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10753-8. doi: 10.1073/pnas.172117099. Epub 2002 Jul 29.
Strains of Toxoplasma gondii can be grouped into three predominant clonal lineages with members of the type I group being uniformly lethal in mice. To elucidate the basis of this extreme virulence, a genetic cross was performed between a highly virulent type I strain (GT-1) and a less-virulent type III strain (CTG), and the phenotypes of resulting progeny were analyzed by genetic linkage mapping. Analysis of independent recombinant progeny identified several quantitative trait loci that contributed to acute virulence. A major quantitative trait locus located on chromosome VII accounted for approximately 50% of the virulence phenotype, whereas a minor locus on chromosome IV, linked to the ROP1 gene, accounted for approximately 10%. These loci are conserved in other type I strains, indicating that acute virulence is controlled by discrete genes common to the type I lineage.
刚地弓形虫菌株可分为三个主要的克隆谱系，其中I型菌株的成员对小鼠具有一致的致死性。为了阐明这种极端毒力的基础，在高毒力的I型菌株（GT-1）和低毒力的III型菌株（CTG）之间进行了遗传杂交，并通过遗传连锁图谱分析了所得后代的表型。对独立重组后代的分析确定了几个导致急性毒力的数量性状基因座。位于VII号染色体上的一个主要数量性状基因座约占毒力表型的50%，而与ROP1基因连锁的IV号染色体上的一个次要基因座约占10%。这些基因座在其他I型菌株中是保守的，表明急性毒力由I型谱系共有的离散基因控制。
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