Ota M, Horiuchi S, Obara K
Neuroendocrinology. 1975;18(4):311-21. doi: 10.1159/000122412.
Using borate buffer a substance that suppresses the ovulation induced with PMS and HCG in immature mice was obtained from acetone-defatted bovine pineal powder by an extraction method similar to that used for an extraction of a gonadotropin-inhibiting substance in urine. This gonadotropin inhibitor in the pineal powder differs from melatonin or arginine vasotocin and seems to be different from the water soluble antigonadotropic substance which has been isolated from the bovine and ovine pineal. Partial purification of the gonadotropin inhibitor was accomplished by a Sephadex G-100 column.
使用硼酸盐缓冲液，通过一种类似于从尿液中提取促性腺激素抑制物质的提取方法，从未脱脂的牛松果体粉末中获得了一种能抑制未成熟小鼠中由孕马血清促性腺激素（PMS）和人绒毛膜促性腺激素（HCG）诱导排卵的物质。松果体粉末中的这种促性腺激素抑制剂与褪黑素或精氨酸血管加压素不同，似乎也不同于已从牛和羊松果体中分离出的水溶性抗促性腺物质。通过葡聚糖G - 100柱对促性腺激素抑制剂进行了部分纯化。
