一些肽类和蛋白质类绵羊松果体组分与一种牛松果体E5组分的比较。

Comparison of some peptidic and proteic ovine pineal fractions with a bovine pineal E5 fraction.

作者信息

Noteborn H P, Ebels I, Pévet P, Reinharz A C, Neacşu C, Salemink C A

出版信息

J Neural Transm. 1982;55(1):27-44. doi: 10.1007/BF01243339.

Abstract

Using rather simple and mild extraction and separation methods, three ovine pineal fractions (XM 300 R-PP7.2' and PP7.2 S) were obtained, which contain peptidic/proteic substances and which show fluorescence characteristics of indoles. The ovine fractions were compared with the bovine pineal E-5 fraction. The ovine fractions are chemically sensitive to normal laboratory light and stable in red light (lambda greater than 600 nm). Immunologically, these fractions and the bovine E 5 fraction are stable. From the results of radioimmunological experiments it was concluded that the bovine pineal E 5 fraction as well as the ovine pineal fraction XM 300 R-PP7.2 and PP7.2S may contain (a) peptide(s) ending by the same carboxy terminal tripeptide Pro-Arg-Gly(NH2).

摘要

通过使用相当简单和温和的提取与分离方法，获得了三种绵羊松果体组分（XM 300 R-PP7.2' 和 PP7.2 S），它们含有肽类/蛋白质类物质，并呈现吲哚的荧光特性。将绵羊组分与牛松果体E-5组分进行了比较。绵羊组分对普通实验室光线化学敏感，在红光（波长大于600 nm）下稳定。在免疫方面，这些组分和牛E-5组分是稳定的。从放射免疫实验结果得出结论，牛松果体E-5组分以及绵羊松果体组分XM 300 R-PP7.2和PP7.2S可能含有以相同羧基末端三肽Pro-Arg-Gly(NH2)结尾的一种或多种肽。