Anisimov Sergey V, Tarasov Kirill V, Stern Michael D, Lakatta Edward G, Boheler Kenneth R
Laboratory of Cardiovascular Science, National Institute on Aging, NIH Baltimore, Maryland 21224, USA.
Genomics. 2002 Aug;80(2):213-22. doi: 10.1006/geno.2002.6821.
Transcriptome comparisons facilitate the identification of developmental, aging, and disease-related genes. To quantify the functionally active genome of adult C57BL/6 mouse heart (AMH), we used serial analysis of gene expression (SAGE) to sequence a total of 88,860 tags or 23,941 unique tags. Over 66% of the unique tags matched either known genes or ESTs. Mitochondrial transcripts accounted for 18.7% of the total transcripts, whereas sarcomeric proteins accounted for 3.2% of all tags. After comparison of AMH expression profiles obtained by SAGE and cDNA arrays, we observed numerous quantitative discrepancies (for example: arrays, mt-Co1 > mt-Co2 > mt-Co3; SAGE, mt-Co1 >> mt-Co3 >or= mt-Co2). We carried out quantitative PCR analyses as an independent test of transcript abundance and determined that SAGE yielded quantitatively reliable data. These SAGE results thus represent the first quantitative expression profile of AMH and serve as a reliable transcriptome reference to identify dynamic changes in cardiac gene expression.
转录组比较有助于鉴定与发育、衰老和疾病相关的基因。为了量化成年C57BL/6小鼠心脏（AMH）的功能活跃基因组，我们使用基因表达序列分析（SAGE）对总共88,860个标签或23,941个独特标签进行测序。超过66%的独特标签与已知基因或EST匹配。线粒体转录本占总转录本的18.7%，而肌节蛋白占所有标签的3.2%。在比较通过SAGE和cDNA阵列获得的AMH表达谱后，我们观察到许多定量差异（例如：阵列，mt-Co1>mt-Co2>mt-Co3；SAGE，mt-Co1>>mt-Co3≥mt-Co2）。我们进行了定量PCR分析作为转录本丰度的独立测试，并确定SAGE产生了定量可靠的数据。因此，这些SAGE结果代表了AMH的第一个定量表达谱，并作为鉴定心脏基因表达动态变化的可靠转录组参考。
