Schmedt Auf Der Günne H, Tenhagen B A, Kutzer P, Forderung D, Heuwieser W
Tierklinik für Fortpflanzung, AG Bestandsbetreuung und Qualitätsmanagement, Freie Universität Berlin.
Dtsch Tierarztl Wochenschr. 2002 Jul;109(7):300-5.
Lactoferrin, lysozyme and the lactoperoxidase-thiocyanate-peroxide-system are naturally occurring antimicrobial components of milk. The objective of this study was to examine, whether these components were responsible for negative results, when mastitis milk is cultured microbiologically. Quarter milk samples from 75 cows with clinical mastitis on a dairy farm in Brandenburg were submitted for microbiological culture and analysed for the content and the activities of the three components. Animals from all stages of lactation with clinical mastitis were included in the study. Animals were examined clinically and milk samples were collected prior to first treatment. Secretions from quarters with clinical mastitis were compared to those of neighbouring quarters without clinical mastitis. Secretions with positive cultural results were compared to those with negative results. The concentrations or activities of the three factors were significantly higher in the diseased quarters than in the quarters without clinical signs of mastitis. The concentration of lysozyme increased with severity of the clinical signs (local swelling and changes in secretion). The concentration of lactoferrin was significantly higher in quarters with slight alterations of glandular tissue than in quarters with medium or severe alterations (P < 0.05). LPS-activities did not correlate with the severity of clinical signs. No differences in the concentration of lactoferrin or LPS-activities were seen between mastitis with positive and negative culture results. The concentration of lysozyme was even higher in culturally positive samples than in negative samples (P < 0.05). Results from this study indicate that the three factors examined did not impair the results of microbiological culture of milk samples from quarters with clinical mastitis.
乳铁蛋白、溶菌酶和乳过氧化物酶-硫氰酸盐-过氧化物系统是牛奶中天然存在的抗菌成分。本研究的目的是检验当对乳腺炎乳汁进行微生物培养时，这些成分是否是导致阴性结果的原因。从勃兰登堡一个奶牛场的75头患有临床乳腺炎的奶牛身上采集乳区乳汁样本，进行微生物培养，并分析这三种成分的含量和活性。研究纳入了处于泌乳各阶段且患有临床乳腺炎的动物。在首次治疗前对动物进行临床检查并采集乳汁样本。将患有临床乳腺炎乳区的分泌物与相邻无临床乳腺炎乳区的分泌物进行比较。将培养结果为阳性的分泌物与阴性分泌物进行比较。患病乳区中这三种因素的浓度或活性显著高于无乳腺炎临床症状的乳区。溶菌酶的浓度随着临床症状（局部肿胀和分泌物变化）的严重程度而增加。乳腺组织轻度改变的乳区中乳铁蛋白的浓度显著高于中度或重度改变的乳区（P<0.05）。脂多糖活性与临床症状的严重程度无关。培养结果为阳性和阴性的乳腺炎之间，乳铁蛋白浓度或脂多糖活性没有差异。培养结果为阳性的样本中溶菌酶的浓度甚至高于阴性样本（P<0.05）。本研究结果表明，所检测的这三种因素并未影响来自患有临床乳腺炎乳区乳汁样本的微生物培养结果。
