Ateya Ahmed, Safhi Fatmah A, El-Emam Huda, Al-Ghadi Muath Q, Abdo Mohamed, Fericean Liana, Olga Rada, Mihaela Ostan, Hizam Manar M, Mamdouh Maha, Abu El-Naga Eman M, Raslan Walaa S
Department of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Vet Sci. 2023 Sep 14;10(9):573. doi: 10.3390/vetsci10090573.
The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo's jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (, , , , , , , and ) genes' nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher's exact test ( ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers' nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy.
本研究检测了可能与意大利水牛产后炎症性疾病相互作用的免疫基因。总共使用了120头意大利雌性水牛（60头正常，60头患有炎症性生殖疾病）。穿刺每头水牛的颈静脉采集5毫升血液。为了获得全血并提取DNA和RNA，将血液置于含有氟化钠或乙二胺四乙酸抗凝剂的试管中。通过PCR-DNA测序发现了健康水牛和患有炎症性生殖疾病的水牛之间免疫基因（、、、、、、和）的核苷酸序列差异。根据Fisher精确检验（˂0.01），所有主要核苷酸变化在有生殖问题和无生殖问题的水牛群体中传播的概率存在显著差异。当水牛患有炎症性生殖疾病时，它们表达所检测基因的可能性显著更高。这些结果可能支持这些标志物的核苷酸变异和基因表达模式作为炎症性生殖障碍患病率指标的重要性，并提供可行的水牛管理策略。
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