Hoshino Yasutaka, Jones Ronald W, Kapikian Albert Z
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8026, USA.
Virology. 2002 Jul 20;299(1):64-71. doi: 10.1006/viro.2002.1474.
Neutralization specificities of outer capsid spike protein VP4 of murine rotavirus strains EW (P?[16],G3) and EHP (P?[20],G3) and lapine rotavirus strains Ala (P?[14],G3), C11 (P?[14],G3), and R2 (P?[14],G3) as well as human rotavirus strains PA169 (P?[14],G6) and HAL1166 (P?[14],G8) were determined by two-way cross-neutralization. This was done by generating and characterizing (i) three murine x human, three lapine x human, and two human x human single gene substitution reassortant rotaviruses, each of which bore identical human rotavirus DS-1 strain VP7 (G2), and (ii) guinea pig hyperimmune antiserum raised against each reassortant. Reference rotavirus strains employed in the study represented 10 established VP4 (P) serotypes, including 1A[8], 1B[4], 2A[6], 3[9], 4[10], 5A[2], 5B[2], 5B[3], 6[1], 7[5], 8[11], 9[7], and 10[16] as well as a P serotype unknown P[18]. Murine rotavirus strains EW and EB were demonstrated to share the same P serotype (P10[16]) distinct from (i) 9 established P serotypes, (ii) lapine and human rotavirus strains bearing the P[14] genotype, and (iii) an equine rotavirus strain bearing the P[18] genotype. Both lapine (Ala, C11, and R2) and human (PA169 and HAL1166) rotaviruses were shown to belong to the same VP4 serotype, which represented a distinct new P serotype (P11[14]). P serotype 13[20] was assigned to murine rotavirus EHP strain VP4, which was shown to be distinct from all the P serotypes/genotypes examined in the present study.
通过双向交叉中和试验确定了鼠轮状病毒EW株（P?[16]，G3）、EHP株（P?[20]，G3）、兔轮状病毒Ala株（P?[14]，G3）、C11株（P?[14]，G3）和R2株（P?[14]，G3）以及人轮状病毒PA169株（P?[14]，G6）和HAL1166株（P?[14]，G8）外衣壳刺突蛋白VP4的中和特异性。具体做法是：（i）构建并鉴定三种鼠-人、三种兔-人以及两种人-人单基因替换重配轮状病毒，每种重配病毒均携带相同的人轮状病毒DS-1株VP7（G2）；（ii）制备针对每种重配病毒的豚鼠超免疫抗血清。本研究中使用的参考轮状病毒株代表10种已确定的VP4（P）血清型，包括1A[8]、1B[4]、2A[6]、3[9]、4[10]、5A[2]、5B[2]、5B[3]、6[1]、7[5]、8[11]、9[7]和10[16]，以及一种血清型未知的P[18]。结果表明，鼠轮状病毒EW株和EB株具有相同的P血清型（P10[16]），与以下几种情况不同：（i）9种已确定的P血清型；（ii）携带P[14]基因型的兔和人轮状病毒株；（iii）携带P[18]基因型的马轮状病毒株。兔（Ala、C11和R2）和人（PA169和HAL1166）轮状病毒均属于同一VP4血清型，代表一种独特的新P血清型（P11[14]）。P血清型13[20]被指定为鼠轮状病毒EHP株的VP4血清型，结果表明它与本研究中检测的所有P血清型/基因型均不同。
