Hoshino Y, Jones R W, Kapikian A Z
Epidemiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
Arch Virol. 1998;143(6):1233-44. doi: 10.1007/s007050050371.
The vervet monkey rotavirus SA11, a prototype strain of group A rotaviruses, has been shown to possess VP7 serotype 3 specificity but its neutralization specificity with regard to the other outer capsid protein VP4 has not been elucidated. We thus determined its VP4 specificity by two-way cross-neutralization with guinea pig antiserum prepared with a single gene substitution reassortant that had only the VP4-encoding gene from the simian rotavirus SA11 strain and remaining ten genes from human rotavirus DS-1 strain (G serotype 2). The SA11 VP4 was related antigenically in a one-way fashion to rhesus monkey rotavirus MMU18006 VP4 (a P5B strain) and marginally to human and canine rotavirus VP4s with P serotype 5A specificity. In addition, the SA11 VP4 was shown to be distinct antigenically from those of other known P serotypes (1-4, and 6-11) as well as those of uncharacterized equine, lapine, and avian rotavirus strains. The SA11 VP4 is thus proposed for classification as a P5B serotype.
黑长尾猴轮状病毒SA11是A组轮状病毒的原型株，已被证明具有VP7血清型3特异性，但关于另一种外衣壳蛋白VP4的中和特异性尚未阐明。因此，我们用豚鼠抗血清进行双向交叉中和试验来确定其VP4特异性，该抗血清是用一种单基因替代重配株制备的，该重配株仅具有来自猿猴轮状病毒SA11株的VP4编码基因，其余十个基因来自人轮状病毒DS-1株（G血清型2）。SA11 VP4在抗原性上与恒河猴轮状病毒MMU18006 VP4（P5B株）呈单向相关，与具有P血清型5A特异性的人和犬轮状病毒VP4呈微弱相关。此外，SA11 VP4在抗原性上与其他已知P血清型（1-4和6-11）以及未鉴定的马、兔和禽轮状病毒株的VP4不同。因此，SA11 VP4被提议归类为P5B血清型。
