[用微量薄层色谱法对血清中总脂质及其组分进行快速微量分析]

[Express microanalysis of total lipids and their fractions in blood serum by micro-thin layer chromatography].

作者信息

Gribanov G A, Sergeev S A

出版信息

Vopr Med Khim. 1975 Nov-Dec;21(6):652-5.

Abstract

A rapid and sensitive microanalysis in blood serum of total lipids and their main fractions (phospholipids, free cholesterol, free fatty acids, triglycerids, esters of cholesterol) by means of micro-thin layer chromatography is described. The quantitative determination of total lipids and their fractions, after elution from the chromatogramms, was carried out by means of degradation with concentrated sulphuric acid and subsequent photometric estimation at 400 nm. The method enabled to determine from 15-20 mcg to 180-200 mcg of total lipids and their fractions. The error of the method did not exceed +/-8.5% for total lipids.

摘要

本文描述了一种通过微量薄层色谱法对血清中总脂质及其主要组分（磷脂、游离胆固醇、游离脂肪酸、甘油三酯、胆固醇酯）进行快速灵敏的微量分析方法。从色谱图上洗脱后，通过浓硫酸降解及随后在400nm处进行光度测定来对总脂质及其组分进行定量测定。该方法能够测定15 - 20微克至180 - 200微克的总脂质及其组分。该方法对总脂质的误差不超过±8.5%。