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[血浆、血清和血凝块中脂质自溶重组的特征]

[Features of autolytic restructuring of lipids in plasma, serum, and blood clots].

作者信息

Gribanov G A, Lutskaia N V

出版信息

Vopr Med Khim. 1993 May-Jun;39(3):12-4.

PMID:8333177
Abstract

Quantitative composition and amount of main lipid fractions were studied by means of microthin-layer chromatography in blood clot free of cell elements, in blood serum and plasma obtained from volunteers as well as their alterations were estimated in dynamics of autolysis. Dissimilar alterations were detected in total lipids and their individual fractions in the preparations studied. Under conditions of incubation the lipid component was found to be highly labile in blood plasma and serum, where total content of lipids was distinctly decreased, while the lipids content was not considerably altered in blood clot. Content of phospholipids was most distinctly decreased with simultaneous accumulation of free fatty acids in all the preparations studied. The most considerable transformations were observed within early steps of autolysis. Dissimilar composition and activity of the enzymatic systems involved in biodegradation and biotransformation of lipid components as well as specific metabolic relations between lipids may be responsible for these differences in the type and rate of autolytic transformations of lipid components in blood plasma, serum and clot.

摘要

采用微薄层色谱法研究了取自志愿者的无细胞成分血凝块、血清和血浆中主要脂质组分的定量组成和含量,并评估了自溶动态过程中的变化。在所研究的制剂中,总脂质及其各个组分出现了不同的变化。在孵育条件下,发现血浆和血清中的脂质成分高度不稳定,脂质总含量明显降低,而血凝块中的脂质含量变化不大。在所有研究的制剂中,磷脂含量明显降低,同时游离脂肪酸积累。自溶早期阶段观察到最显著的变化。参与脂质成分生物降解和生物转化的酶系统的不同组成和活性以及脂质之间的特定代谢关系,可能是血浆、血清和血凝块中脂质成分自溶转化类型和速率存在这些差异的原因。

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