Chambers Geoffrey K, Marshall Stephen J, Robinson Geoffrey M, Maguire Sean, Newton-Howes Jan, Chong Nicola L
School of Biological Sciences, Victoria University, Wellington, New Zealand.
Alcohol Clin Exp Res. 2002 Jul;26(7):949-55. doi: 10.1097/01.ALC.0000021145.47616.38.
The last 10 years have seen growing recognition of the significance of the genes encoding enzymes responsible for hepatic alcohol metabolism as protective factors in the development of alcoholism.
We have developed DNA sequencing assays for measuring genetic variation at the alcohol dehydrogenase 2 (ADH2), ADH3, and aldehyde dehydrogenase 2 (ALDH2) loci. These have been used to survey volunteer control subjects from three New Zealand ethnic groups (white, Asian, and Polynesian) and young male alcoholics recruited from white and New Zealand Maori patients in a local treatment program.
The allele frequency values for whites and Asians obtained in our study closely match those obtained previously in other laboratories. Our data (the first for Polynesians) are 0.42 for ADH22, 0.78 for ADH31, and 0.00 for ALDH22. In the New Zealand Maori alcoholic patients, the ADH22 frequency is significantly lower (0.15; p < 0.01). The frequency of ADH3*1 is also lower in this group (0.60), but this value is not significant (0.05 < p < 0.06).
In young male New Zealand Maori, the ADH22 allele is a protective factor against alcoholism even in the absence of ALDH22.
在过去十年中，人们越来越认识到编码负责肝脏酒精代谢的酶的基因作为酒精中毒发展中的保护因素的重要性。
我们开发了用于测量酒精脱氢酶2（ADH2）、ADH3和乙醛脱氢酶2（ALDH2）基因座遗传变异的DNA测序分析方法。这些方法已用于调查来自三个新西兰种族群体（白人、亚洲人和波利尼西亚人）的志愿者对照受试者，以及从当地治疗项目中的白人和新西兰毛利患者招募的年轻男性酗酒者。
我们研究中获得的白人和亚洲人的等位基因频率值与其他实验室先前获得的值密切匹配。我们的数据（波利尼西亚人的首个数据）显示，ADH22为0.42，ADH31为0.78，ALDH22为0.00。在新西兰毛利酗酒患者中，ADH22频率显著较低（0.15；p<0.01）。该组中ADH3*1的频率也较低（0.60），但该值不显著（0.05<p<0.06）。
在年轻男性新西兰毛利人中，即使没有ALDH22，ADH22等位基因也是预防酒精中毒的保护因素。
